Abstract
At 5 degrees C four strains of fermentative, gram-negative bacteria (Serratia liquefaciens, Yersinia enterocolitica, Enterobacter cloacae, and Aeromonas hydrophila) grew aerobically and anaerobically on adipose tissue removed from beef muscle of low pH (5.4 to 5.6). All four strains also grew aerobically and anaerobically on muscle tissue of high pH (6.0 to 6.3). However, none of the four grew anaerobically on beef muscle of low pH, and the aeromonad strain also failed to grow aerobically on such muscle. Growth of S. liquefaciens and E.cloacae on vacuum-packaged beef muscle was dependent on the pH of the tissue and the oxygen transmission rate of the packaging film. Although the four strains grew in broth buffered at pH 5.55, L-lactate, at the concentration found in muscle of low pH (ca. 100 mM), prevented anaerobic growth of all four isolates and prevented the aerobic growth of th aeromonad. At pH 6.1 in buffered broth, the concentration of L-lactate occurring in muscle of high pH did not prevent aerobic or anaerobic growth of any of the strains.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Campbell R. J., Egan A. F., Grau F. H., Shay B. J. The growtn of Microbacterium thermosphactum on beef. J Appl Bacteriol. 1979 Dec;47(3):505–509. doi: 10.1111/j.1365-2672.1979.tb01211.x. [DOI] [PubMed] [Google Scholar]
- Gill C. O., Newton K. G. Spoilage of vacuum-packaged dark, firm, dry meat at chill temperatures. Appl Environ Microbiol. 1979 Mar;37(3):362–364. doi: 10.1128/aem.37.3.362-364.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gill C. O. Substrate limitation of bacterial growth at meat surfaces. J Appl Bacteriol. 1976 Dec;41(3):401–410. doi: 10.1111/j.1365-2672.1976.tb00652.x. [DOI] [PubMed] [Google Scholar]
- Grau F. H. Inhibition of the Anaerobic Growth of Brochothrix thermosphacta by Lactic Acid. Appl Environ Microbiol. 1980 Sep;40(3):433–436. doi: 10.1128/aem.40.3.433-436.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Newton K. G., Gill C. O. The development of the anaerobic spoilage flora of meat stored at chill temperatures. J Appl Bacteriol. 1978 Feb;44(1):91–95. doi: 10.1111/j.1365-2672.1978.tb00779.x. [DOI] [PubMed] [Google Scholar]
- Popoff M., Véron M. A taxonomic study of the Aeromonas hydrophila-Aeromonas punctata group. J Gen Microbiol. 1976 May;94(1):11–22. doi: 10.1099/00221287-94-1-11. [DOI] [PubMed] [Google Scholar]
- Seelye R. J., Yearbury B. J. Isolation of Yersinia enterocolitica-resembling organisms and Alteromonas putrefaciens from vacuum-packed chilled beef cuts. J Appl Bacteriol. 1979 Jun;46(3):493–499. doi: 10.1111/j.1365-2672.1979.tb00848.x. [DOI] [PubMed] [Google Scholar]