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. 1982 May;43(5):1020–1025. doi: 10.1128/aem.43.5.1020-1025.1982

Effect of free-radical scavengers on enumeration of thermally stressed cells of Staphylococcus aureus MF-31.

E R Bucker, S E Martin
PMCID: PMC244180  PMID: 6285821

Abstract

Exposure of crude cell lysates of Staphylococcus aureus MF-31 to 5 or 10 mM hydrogen peroxide resulted in a linear decrease in superoxide dismutase activity. Approximately 13% of the superoxide dismutase activity was lost after 16 min. Thermally stressed and nonstressed cells were exposed to a photochemically generated exogenous flux of superoxide radicals (O2.-). The death of thermally stressed cells was linear with time. Addition of superoxide dismutase or catalase to the O2.- generating system resulted in protection of thermally stressed and nonstressed cells, with the protective effect being greater for thermally stressed cells. Incorporation of O2-, hydroxyl radical, or singlet oxygen scavengers or antioxidants to tryptic soy agar containing 7.5% NaCl did not increase the enumeration of thermally stressed cells.

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Selected References

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