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. 1982 May;43(5):1196–1200. doi: 10.1128/aem.43.5.1196-1200.1982

Flagella and Pili of Iron-Oxidizing Thiobacilli Isolated from a Uranium Mine in Northern Ontario, Canada

Alan A DiSpirito 1, Marvin Silver 2, Lance Voss 1, Olli H Tuovinen 1
PMCID: PMC244205  PMID: 16346016

Abstract

Five strains of Thiobacillus ferrooxidans, which included three recent isolates from a uranium mine, possessed flagella. Three of the strains had several pili per cell. The dimensions, fine structure, and orientation of the flagella were different. Both polar and peritrichous flagella were observed, indicating strain-dependent ultrastructural variation in acidophilic thiobacilli. Neither flagella nor pili were detected in eight other strains of T. ferrooxidans and two strains of Thiobacillus acidophilus by electron microscopy, although all of the cultures contained motile cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRALEY S. A., Sr, KINSEL N. A., LEATHEN W. W. Ferrobacillus ferrooxidans: a chemosynthetic autotrophic Bacterium. J Bacteriol. 1956 Nov;72(5):700–704. doi: 10.1128/jb.72.5.700-704.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Doetsch R. N., Cook T. M., Vaituzis Z. On the uniqueness of the flagellum of Thiobacillus thiooxidans. Antonie Van Leeuwenhoek. 1967;33(2):196–202. doi: 10.1007/BF02045551. [DOI] [PubMed] [Google Scholar]
  3. Gromova L. A., Pereverzev N. A., Karavaiko G. I. Pili Thiobacillus ferrooxidans. Mikrobiologiia. 1978 Mar-Apr;47(2):293–295. [PubMed] [Google Scholar]
  4. Guay R., Silver M. Thiobacillus acidophilus sp. nov.; isolation and some physiological characteristics. Can J Microbiol. 1975 Mar;21(3):281–288. doi: 10.1139/m75-040. [DOI] [PubMed] [Google Scholar]
  5. Guay R., Silver M., Torma A. E. Base competition of DNA isolated from Thiobacillus ferrooxidans grown on different substrates. Rev Can Biol. 1976 Jun;35(2):61–67. [PubMed] [Google Scholar]
  6. Martin P. A., Dugan P. R., Tuovinen O. H. Plasmid DNA in acidophilic, chemolithotrophic thiobacilli. Can J Microbiol. 1981 Aug;27(8):850–853. doi: 10.1139/m81-133. [DOI] [PubMed] [Google Scholar]
  7. Reynolds D. M., Laishley E. J., Costerton J. W. Physiological and ultrastructural characterization of a new acidophilic Thiobacillus species (T. kabobis). Can J Microbiol. 1981 Feb;27(2):151–161. doi: 10.1139/m81-025. [DOI] [PubMed] [Google Scholar]
  8. Silver M., Torma A. E. Oxidation of metal sulfides by Thiobacillus ferrooxidans grown on different substrates. Can J Microbiol. 1974 Feb;20(2):141–147. doi: 10.1139/m74-023. [DOI] [PubMed] [Google Scholar]
  9. Tuovinen O. H., Kelly D. P. Studies on the growth of Thiobacillus ferrooxidans. I. Use of membrane filters and ferrous iron agar to determine viable numbers, and comparison with 14 CO 2 -fixation and iron oxidation as measures of growth. Arch Mikrobiol. 1973;88(4):285–298. [PubMed] [Google Scholar]
  10. Tuovinen O. H., Kelly D. P. Studies on the growth of Thiobacillus ferrooxidans. V. Factors affecting growth in liquid culture and development of colonies on solid media containing inorganic sulphur compounds. Arch Microbiol. 1974 Jul 22;98(4):351–364. doi: 10.1007/BF00425295. [DOI] [PubMed] [Google Scholar]
  11. Wichlacz P. L., Unz R. F. Acidophilic, heterotrophic bacteria of acidic mine waters. Appl Environ Microbiol. 1981 May;41(5):1254–1261. doi: 10.1128/aem.41.5.1254-1261.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

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