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. 1982 Jun;43(6):1498–1500. doi: 10.1128/aem.43.6.1498-1500.1982

Asporogenous Bacillus thuringiensis Mutant Producing High Yields of δ-Endotoxin

Yoshiharu Wakisaka 1, Emiko Masaki 1, Kenzo Koizumi 1, Yoji Nishimoto 1, Yasuhisa Endo 1, Masaji S Nishimura 1, Junko Nishiitsutsuji-Uwo 1
PMCID: PMC244259  PMID: 16346044

Abstract

An asporogenous Bacillus thuringiensis subsp. kurstaki strain IK mutant, strain 290-1, which produced high yields of δ-endotoxin, was obtained by ethyl methane sulfonate treatment of a spore suspension. The mutant strain produced about the same amount of δ-endotoxin as that produced by the parent strain, but 1010 to 1011 cells did not form any detectable dormant spores.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dulmage H. T. Production of -endotoxin by eighteen isolates of Bacillus thuringiensis, serotype 3, in 3 fermentation media. J Invertebr Pathol. 1971 Nov;18(3):353–358. doi: 10.1016/0022-2011(71)90037-1. [DOI] [PubMed] [Google Scholar]
  2. Ito J., Spizizen J. Increased rate of asporogenous mutations following treatment of Bacillus subtilis spores with ethyl methanesulfonate. Mutat Res. 1971 Sep;13(1):93–96. doi: 10.1016/0027-5107(71)90130-8. [DOI] [PubMed] [Google Scholar]
  3. Johnson D. E., Niezgodski D. M., Twaddle G. M. Parasporal crystals produced by oligosporogenous mutans of Bacillus thuringiensis (Spo-Cr+). Can J Microbiol. 1980 Apr;26(4):486–491. doi: 10.1139/m80-081. [DOI] [PubMed] [Google Scholar]
  4. Somerville H. J. Formation of the parasporal inclusion of Bacillus thuringiensis. Eur J Biochem. 1971 Jan;18(2):226–237. doi: 10.1111/j.1432-1033.1971.tb01235.x. [DOI] [PubMed] [Google Scholar]
  5. Yousten A. A. A method for the isolation of asporogenic mutants of Bacillus thuringiensis. Can J Microbiol. 1978 Apr;24(4):492–494. doi: 10.1139/m78-081. [DOI] [PubMed] [Google Scholar]

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