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. 2003 Oct;4(5):525–530. doi: 10.1002/cfg.326

Padlock and Proximity Probes for in Situ and Array-Based Analyses: Tools for the Post-Genomic Era

Ulf Landegren 1,✉, Fredrik Dahl 1, Mats Nilsson 1, Simon Fredriksson 1, Johan Banér 1, Mats Gullberg 1, Jonas Jarvius 1, Sigrun Gustafsdottir 1, Ola Söderberg 1, Olle Ericsson 1, Johan Stenberg 1, Edith Schallmeiner 1
PMCID: PMC2447294  PMID: 18629012

Abstract

Highly specific high-throughput assays will be required to take full advantage of the accumulating information about the macromolecular composition of cells and tissues, in order to characterize biological systems in health and disease. We discuss the general problem of detection specificity and present the approach our group has taken, involving the reformatting of analogue biological information to digital reporter segments of genetic information via a series of DNA ligation assays. The assays enable extensive, coordinated analyses of the numbers and locations of genes, transcripts and protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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