Abstract
To date, the overwhelming majority of genomics programs in plants have been directed at model or crop plant species, meaning that very little of the naturally occurring sequence diversity found in plants is available for characterization and exploitation. In contrast, ‘xenogenomics’ refers to the discovery and functional analysis of novel genes and alleles from indigenous and exotic species, permitting bioprospecting of biodiversity using high-throughput genomics experimental approaches. Such a program has been initiated to bioprospect for genetic determinants of abiotic stress tolerance in indigenous Australian flora and native Antarctic plants. Uniquely adapted Poaceae and Fabaceae species with enhanced tolerance to salt, drought, elevated soil aluminium concentration, and freezing stress have been identified, based primarily on their eco-physiology, and have been subjected to structural and functional genomics analyses. For each species, EST collections have been derived from plants subjected to appropriate abiotic stresses. Transcript profiling with spotted unigene cDNA micro-arrays has been used to identify genes that are transcriptionally modulated in response to abiotic stress. Candidate genes identified on the basis of sequence annotation or transcript profiling have been assayed in planta and other in vivo systems for their capacity to confer novel phenotypes. Comparative genomics analysis of novel genes and alleles identified in the xenogenomics target plant species has subsequently been undertaken with reference to key model and crop plants.
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Selected References
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- Kore-eda Shin, Cushman Mary Ann, Akselrod Inna, Bufford Davina, Fredrickson Monica, Clark Elizabeth, Cushman John C. Transcript profiling of salinity stress responses by large-scale expressed sequence tag analysis in Mesembryanthemum crystallinum. Gene. 2004 Oct 27;341:83–92. doi: 10.1016/j.gene.2004.06.037. [DOI] [PubMed] [Google Scholar]
- Rudd Stephen. openSputnik--a database to ESTablish comparative plant genomics using unsaturated sequence collections. Nucleic Acids Res. 2005 Jan 1;33(DATABASE):D622–D627. doi: 10.1093/nar/gki040. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sidebottom C., Buckley S., Pudney P., Twigg S., Jarman C., Holt C., Telford J., McArthur A., Worrall D., Hubbard R. Heat-stable antifreeze protein from grass. Nature. 2000 Jul 20;406(6793):256–256. doi: 10.1038/35018639. [DOI] [PubMed] [Google Scholar]
- Tomczak Melanie M., Marshall Christopher B., Gilbert Jack A., Davies Peter L. A facile method for determining ice recrystallization inhibition by antifreeze proteins. Biochem Biophys Res Commun. 2003 Nov 28;311(4):1041–1046. doi: 10.1016/j.bbrc.2003.10.106. [DOI] [PubMed] [Google Scholar]