Abstract
Circulating antigliadin antibody has been described in patients with gluten enteropathy although the prevalence varies in different studies. It has been suggested that the investigation for antigliadin antibody might be useful as a screening test. The object of the present study was to evaluate two different techniques for assaying these antibodies — an indirect immunofluorescent method and an enzyme-linked immunosorbent assay (ELISA). Antibodies were assayed in the sera of 102 patients in whom jejunal biopsies were also obtained. The specificity of both tests was greater than 95%, and the correlation between the presence of antibody and histology was significant (p < 0.005), though the sensitivity of each test was less than 70%.
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Selected References
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- Eterman K. P., Feltkamp T. E. Antibodies to gluten and reticulin in gastrointestinal diseases. Clin Exp Immunol. 1978 Jan;31(1):92–99. [PMC free article] [PubMed] [Google Scholar]
- Kelly J., O'Farrelly C., Rees J. P., Feighery C. F., Weir D. G. Alpha-gliadin antibodies in childhood coeliac disease. Lancet. 1985 Sep 7;2(8454):558–559. doi: 10.1016/s0140-6736(85)90502-1. [DOI] [PubMed] [Google Scholar]
- O'Farrelly C., Kelly J., Hekkens W., Bradley B., Thompson A., Feighery C., Weir D. G. Alpha gliadin antibody levels: a serological test for coeliac disease. Br Med J (Clin Res Ed) 1983 Jun 25;286(6383):2007–2010. doi: 10.1136/bmj.286.6383.2007. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Savilahti E., Viander M., Perkkiö M., Vainio E., Kalimo K., Reunala T. IgA antigliadin antibodies: a marker of mucosal damage in childhood coeliac disease. Lancet. 1983 Feb 12;1(8320):320–322. doi: 10.1016/s0140-6736(83)91627-6. [DOI] [PubMed] [Google Scholar]
- Stern M., Fischer K., Grüttner R. Immunofluorescent serum gliadin antibodies in children with coeliac disease and various malabsorptive disorders. I. Technique, clinical evaluation and diagnostic use of a gliadin antibody assay using pyruvic aldehyde-treated human red cells. Eur J Pediatr. 1979 Mar 1;130(3):155–164. doi: 10.1007/BF00455261. [DOI] [PubMed] [Google Scholar]
- Unsworth D. J., Kieffer M., Holborow E. J., Coombs R. R., Walker-Smith J. A. IgA anti-gliadin antibodies in coeliac disease. Clin Exp Immunol. 1981 Nov;46(2):286–293. [PMC free article] [PubMed] [Google Scholar]
- Unsworth D. J., Manuel P. D., Walker-Smith J. A., Campbell C. A., Johnson G. D., Holborow E. J. New immunofluorescent blood test for gluten sensitivity. Arch Dis Child. 1981 Nov;56(11):864–868. doi: 10.1136/adc.56.11.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Volta U., Lenzi M., Lazzari R., Cassani F., Collina A., Bianchi F. B., Pisi E. Antibodies to gliadin detected by immunofluorescence and a micro-ELISA method: markers of active childhood and adult coeliac disease. Gut. 1985 Jul;26(7):667–671. doi: 10.1136/gut.26.7.667. [DOI] [PMC free article] [PubMed] [Google Scholar]