Abstract
Two polymorphisms in the IL4 (G/C 3′-UTR) and IL5 (C-703T) genes were studied in a sample of families whose probands had atopic bronchial asthma (BA) (66 families, n = 183) and in a group of non-cognate individuals with the severe form of the disease (n = 34). The samples were collected from the Russian population in the city of Tomsk (Russia). Using the transmission/disequilibrium test (TDT), a significant association of allele C-703 IL5 with BA was established (TDT = 4.923, p = 0.007 ± 0.0007). The analysis of 40 individuals with mild asthma and 49 patients with the severe form of the disease revealed a negative association of genotype GG IL4 (OR = 0.39, 95% CI = 0.15−0.99, p = 0.035), and also a trend towards a positive association of the GC IL4 genotype (OR = 2.52, 95% CI = 0.98−6.57, p = 0.052) with mild BA. There was a concordance of the clinical classification of BA severity with the ‘genotype’ (McNemar’s χ2 test with continuity correction constituted 0.03, d.f. = 1, p = 0.859). These results suggest that polymorphisms in the IL4 and IL5 genes contribute to the susceptibility to atopic BA and could determine the clinical course of the disease.
Full Text
The Full Text of this article is available as a PDF (101.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chung K. F., Barnes P. J. Cytokines in asthma. Thorax. 1999 Sep;54(9):825–857. doi: 10.1136/thx.54.9.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cookson W. O., Moffatt M. F. Genetics of asthma and allergic disease. Hum Mol Genet. 2000 Oct;9(16):2359–2364. doi: 10.1093/hmg/9.16.2359. [DOI] [PubMed] [Google Scholar]
- Paul W. E., Seder R. A. Lymphocyte responses and cytokines. Cell. 1994 Jan 28;76(2):241–251. doi: 10.1016/0092-8674(94)90332-8. [DOI] [PubMed] [Google Scholar]
- Pereira E., Goldblatt J., Rye P., Sanderson C., Le Souëf P. Mutation analysis of interleukin-5 in an asthmatic cohort. Hum Mutat. 1998;11(1):51–54. doi: 10.1002/(SICI)1098-1004(1998)11:1<51::AID-HUMU8>3.0.CO;2-O. [DOI] [PubMed] [Google Scholar]
- Rioux J. D., Stone V. A., Daly M. J., Cargill M., Green T., Nguyen H., Nutman T., Zimmerman P. A., Tucker M. A., Hudson T. Familial eosinophilia maps to the cytokine gene cluster on human chromosomal region 5q31-q33. Am J Hum Genet. 1998 Oct;63(4):1086–1094. doi: 10.1086/302053. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spielman R. S., McGinnis R. E., Ewens W. J. Transmission test for linkage disequilibrium: the insulin gene region and insulin-dependent diabetes mellitus (IDDM). Am J Hum Genet. 1993 Mar;52(3):506–516. [PMC free article] [PubMed] [Google Scholar]
- Stavnezer J. Antibody class switching. Adv Immunol. 1996;61:79–146. doi: 10.1016/s0065-2776(08)60866-4. [DOI] [PubMed] [Google Scholar]