Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1991 Jan;35(1):185–187. doi: 10.1128/aac.35.1.185

Inducible transfer of conjugative transposon Tn1545 from Enterococcus faecalis to Listeria monocytogenes in the digestive tracts of gnotobiotic mice.

F Doucet-Populaire 1, P Trieu-Cuot 1, I Dosbaa 1, A Andremont 1, P Courvalin 1
PMCID: PMC244965  PMID: 1849709

Abstract

Transfer of conjugative transposon Tn1545 from Enterococcus faecalis to Listeria monocytogenes was studied in vitro and in vivo. Tn1545 transferred following filter mating at a frequency of 2.5 x 10(-7) transconjugants per donor colony. A 20-fold increase in transfer frequency was observed when matings were performed in the presence of a subinhibitory concentration of tetracycline. The frequency of in vivo transfer of Tn1545, expressed as the number of transconjugants per donor cell extracted from the intestines of the gnotobiotic mice after 35 days of experiment, was 1.1 x 10(-8). Presence of a low concentration of tetracycline in the drinking water increased this frequency 10-fold.

Full text

PDF
185

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andremont A., Gerbaud G., Tancrède C., Courvalin P. Plasmid-mediated susceptibility to intestinal microbial antagonisms in Escherichia coli. Infect Immun. 1985 Sep;49(3):751–755. doi: 10.1128/iai.49.3.751-755.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Andremont A., Raibaud P., Tancrède C. Effect of erythromycin on microbial antagonisms: a study in gnotobiotic mice associated with a human fecal flora. J Infect Dis. 1983 Sep;148(3):579–587. doi: 10.1093/infdis/148.3.579. [DOI] [PubMed] [Google Scholar]
  3. Courvalin P., Carlier C. Transposable multiple antibiotic resistance in Streptococcus pneumoniae. Mol Gen Genet. 1986 Nov;205(2):291–297. doi: 10.1007/BF00430441. [DOI] [PubMed] [Google Scholar]
  4. Freter R., Brickner H., Fekete J., Vickerman M. M., Carey K. E. Survival and implantation of Escherichia coli in the intestinal tract. Infect Immun. 1983 Feb;39(2):686–703. doi: 10.1128/iai.39.2.686-703.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gellin B. G., Broome C. V. Listeriosis. JAMA. 1989 Mar 3;261(9):1313–1320. [PubMed] [Google Scholar]
  6. Ivins B. E., Welkos S. L., Knudson G. B., Leblanc D. J. Transposon Tn916 mutagenesis in Bacillus anthracis. Infect Immun. 1988 Jan;56(1):176–181. doi: 10.1128/iai.56.1.176-181.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Poyart-Salmeron C., Carlier C., Trieu-Cuot P., Courtieu A. L., Courvalin P. Transferable plasmid-mediated antibiotic resistance in Listeria monocytogenes. Lancet. 1990 Jun 16;335(8703):1422–1426. doi: 10.1016/0140-6736(90)91447-i. [DOI] [PubMed] [Google Scholar]
  8. Poyart-Salmeron C., Trieu-Cuot P., Carlier C., Courvalin P. Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545: homologies with other site-specific recombinases. EMBO J. 1989 Aug;8(8):2425–2433. doi: 10.1002/j.1460-2075.1989.tb08373.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pérez-Díaz J. C., Vicente M. F., Baquero F. Plasmids in Listeria. Plasmid. 1982 Sep;8(2):112–118. doi: 10.1016/0147-619x(82)90049-x. [DOI] [PubMed] [Google Scholar]
  10. Trieu-Cuot P., Carlier C., Courvalin P. Conjugative plasmid transfer from Enterococcus faecalis to Escherichia coli. J Bacteriol. 1988 Sep;170(9):4388–4391. doi: 10.1128/jb.170.9.4388-4391.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES