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. 1991 Mar;35(3):575–578. doi: 10.1128/aac.35.3.575

Nonradioactive DNA probe for detection of gene for methicillin resistance in Staphylococcus aureus.

M Ligozzi 1, G M Rossolini 1, E A Tonin 1, R Fontana 1
PMCID: PMC245053  PMID: 2039210

Abstract

A DNA probe was developed by inserting, in the SmaI site of pBluescript sK, a 528-bp fragment of the gene responsible for intrinsic resistance to beta-lactam antibiotics in Staphylococcus aureus (mec determinant). The mec probe provided a useful tool for the rapid identification of the intrinsic resistance trait and for establishing guidelines for testing the in vitro susceptibility of S. aureus to beta-lactams.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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