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. 1991 May;35(5):813–818. doi: 10.1128/aac.35.5.813

Role of the beta-lactamase of Campylobacter jejuni in resistance to beta-lactam agents.

N Lachance 1, C Gaudreau 1, F Lamothe 1, L A Larivière 1
PMCID: PMC245112  PMID: 1854162

Abstract

We studied the role of the beta-lactamase of Campylobacter jejuni in resistance to beta-lactam agents. beta-Lactamase-positive strains were more resistant than beta-lactamase-negative strains to amoxicillin, ampicillin, and ticarcillin (P less than 0.05). With penicillin G, piperacillin, imipenem, and six cephalosporins, the susceptibility levels were similar for both beta-lactamase-positive and -negative strains. By using spectrophotometric and microbiological assays, the beta-lactamase from three strains hydrolyzed ampicillin, amoxicillin, penicillin G, cloxacillin, and, partially, cephalothin. Ticarcillin and piperacillin were partially hydrolyzed in the microbiological assay. There was no activity against five other cephalosporins or imipenem. Isoelectric focusing of the enzyme showed a pI of 8.8. Tazobactam was the best inhibitor of the enzyme, followed by clavulanic acid, sulbactam, and cefoxitin, while EDTA and p-chloromercuribenzoate had no activity. All beta-lactamase-positive strains became susceptible to amoxicillin and ampicillin with 1 micrograms of clavulanic acid per ml. With the same inhibitor, there was a reduced but significant effect for ticarcillin but no effect for penicillin G or piperacillin. Sulbactam had no effect and tazobactam was effective only at 2 micrograms/ml on amoxicillin and ampicillin. The beta-lactamase of C. jejuni seems to be a penicillinase with a role in resistance for only amoxicillin, ampicillin, and ticarcillin.

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Selected References

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  1. Ahonkhai V. I., Cherubin C. E., Sierra M. F., Bokkenheuser V. D., Shulman M. A., Mosenthal A. C. In vitro susceptibility of Campylobacter fetus subsp. jejuni to N-formimidoyl thienamycin, rosaramicin, cefoperazone, and other antimicrobial agents. Antimicrob Agents Chemother. 1981 Dec;20(6):850–851. doi: 10.1128/aac.20.6.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aronoff S. C., Jacobs M. R., Johenning S., Yamabe S. Comparative activities of the beta-lactamase inhibitors YTR 830, sodium clavulanate, and sulbactam combined with amoxicillin or ampicillin. Antimicrob Agents Chemother. 1984 Oct;26(4):580–582. doi: 10.1128/aac.26.4.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gaudreau C. L., Lariviere L. A., Lauzer J. C., Turgeon F. F. Effect of clavulanic acid on susceptibility of Campylobacter jejuni and Campylobacter coli to eight beta-lactam antibiotics. Antimicrob Agents Chemother. 1987 Jun;31(6):940–942. doi: 10.1128/aac.31.6.940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Goossens H., De Mol P., Coignau H., Levy J., Grados O., Ghysels G., Innocent H., Butzler J. P. Comparative in vitro activities of aztreonam, ciprofloxacin, norfloxacin, ofloxacin, HR 810 (a new cephalosporin), RU28965 (a new macrolide), and other agents against enteropathogens. Antimicrob Agents Chemother. 1985 Mar;27(3):388–392. doi: 10.1128/aac.27.3.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gutmann L., Ferré B., Goldstein F. W., Rizk N., Pinto-Schuster E., Acar J. F., Collatz E. SHV-5, a novel SHV-type beta-lactamase that hydrolyzes broad-spectrum cephalosporins and monobactams. Antimicrob Agents Chemother. 1989 Jun;33(6):951–956. doi: 10.1128/aac.33.6.951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gutmann L., Kitzis M. D., Yamabe S., Acar J. F. Comparative evaluation of a new beta-lactamase inhibitor, YTR 830, combined with different beta-lactam antibiotics against bacteria harboring known beta-lactamases. Antimicrob Agents Chemother. 1986 May;29(5):955–957. doi: 10.1128/aac.29.5.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karmali M. A., De Grandis S., Fleming P. C. Antimicrobial susceptibility of Campylobacter jejuni and Campylobacter fetus subsp. fetus to eight cephalosporins with special reference to species differentiation. Antimicrob Agents Chemother. 1980 Dec;18(6):948–951. doi: 10.1128/aac.18.6.948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Karmali M. A., De Grandis S., Fleming P. C. Antimicrobial susceptibility of Campylobacter jejuni with special reference to resistance patterns of Canadian isolates. Antimicrob Agents Chemother. 1981 Apr;19(4):593–597. doi: 10.1128/aac.19.4.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kuck N. A., Jacobus N. V., Petersen P. J., Weiss W. J., Testa R. T. Comparative in vitro and in vivo activities of piperacillin combined with the beta-lactamase inhibitors tazobactam, clavulanic acid, and sulbactam. Antimicrob Agents Chemother. 1989 Nov;33(11):1964–1969. doi: 10.1128/aac.33.11.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lariviere L. A., Gaudreau C. L., Turgeon F. F. Susceptibility of clinical isolates of Campylobacter jejuni to twenty-five antimicrobial agents. J Antimicrob Chemother. 1986 Dec;18(6):681–685. doi: 10.1093/jac/18.6.681. [DOI] [PubMed] [Google Scholar]
  11. Michel J., Rogol M., Dickman D. Susceptibility of clinical isolates of Campylobacter jejuni to sixteen antimicrobial agents. Antimicrob Agents Chemother. 1983 May;23(5):796–797. doi: 10.1128/aac.23.5.796. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nikaido H. Outer membrane of Salmonella typhimurium. Transmembrane diffusion of some hydrophobic substances. Biochim Biophys Acta. 1976 Apr 16;433(1):118–132. doi: 10.1016/0005-2736(76)90182-6. [DOI] [PubMed] [Google Scholar]
  13. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Page W. J., Huyer G., Huyer M., Worobec E. A. Characterization of the porins of Campylobacter jejuni and Campylobacter coli and implications for antibiotic susceptibility. Antimicrob Agents Chemother. 1989 Mar;33(3):297–303. doi: 10.1128/aac.33.3.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Severin W. P. Campylobacter en enteritis. Ned Tijdschr Geneeskd. 1978 Apr 15;122(15):499–504. [PubMed] [Google Scholar]
  16. Sykes R. B., Nordström K. Microiodometric determination of beta-lactamase activity. Antimicrob Agents Chemother. 1972 Feb;1(2):94–99. doi: 10.1128/aac.1.2.94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Van der Auwera P., Scorneaux B. In vitro susceptibility of Campylobacter jejuni to 27 antimicrobial agents and various combinations of beta-lactams with clavulanic acid or sulbactam. Antimicrob Agents Chemother. 1985 Jul;28(1):37–40. doi: 10.1128/aac.28.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Vanhoof R., Gordts B., Dierickx R., Coignau H., Butzler J. P. Bacteriostatic and bactericidal activities of 24 antimicrobial agents against Campylobacter fetus subsp. jejuni. Antimicrob Agents Chemother. 1980 Jul;18(1):118–121. doi: 10.1128/aac.18.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vanhoof R., Vanderlinden M. P., Dierickx R., Lauwers S., Yourassowsky E., Butzler J. P. Susceptibility of Campylobacter fetus subsp. jejuni to twenty-nine antimicrobial agents. Antimicrob Agents Chemother. 1978 Oct;14(4):553–556. doi: 10.1128/aac.14.4.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Walder M. Susceptibility of Campylobacter fetus subsp. jejuni to twenty antimicrobiol agents. Antimicrob Agents Chemother. 1979 Jul;16(1):37–39. doi: 10.1128/aac.16.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yoshimura F., Nikaido H. Diffusion of beta-lactam antibiotics through the porin channels of Escherichia coli K-12. Antimicrob Agents Chemother. 1985 Jan;27(1):84–92. doi: 10.1128/aac.27.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]

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