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. 1991 Sep;35(9):1717–1720. doi: 10.1128/aac.35.9.1717

Effects of propicillin on mixed continuous cultures of periodontal bacteria.

J S van der Hoeven 1, C W van den Kieboom 1
PMCID: PMC245256  PMID: 1952836

Abstract

Experiments were designed to test the antibiotic (1-phenoxypropyl)penicillin (propicillin) against a complex microflora of periodontal bacteria. This was accomplished by using a continuously growing mixed culture that was obtained by enrichment of periodontal plaque in human serum. Peptostreptococcus species, Prevotella intermedia, Lactobacillus, catenaforme, and Streptococcus species were dominant members of the enrichment culture. None of the strains isolated from the enrichment culture exhibited detectable beta-lactamase activity. MICs of propicillin for the organisms ranged from 0.1 to 1.2 mg/liter. Propicillin was added to the cultures in single doses that were repeated once or twice at 24-h intervals, that is, after 2.4 volume changes of the culture vessel. Analyses done 24 h after the last addition of propicillin revealed that total cell counts of the culture were hardly affected by 1 mg of propicillin per liter, although some changes in the microbial composition occurred. The relative insusceptibility of the culture might be explained by the low growth rate. Higher concentrations (5, 10, and 50 mg/liter) of the antibiotic caused 10- to 20-fold drops in total cell counts. In these cultures P. intermedia was selectively suppressed to below the detection level, whereas other organisms that were equally susceptible to propicillin were less affected. It was concluded that mixed continuous cultures are a useful tool for studying the effects of antibiotics against the periodontal microbiota.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown M. R., Williams P. Influence of substrate limitation and growth phase on sensitivity to antimicrobial agents. J Antimicrob Chemother. 1985 Jan;15 (Suppl A):7–14. doi: 10.1093/jac/15.suppl_a.7. [DOI] [PubMed] [Google Scholar]
  2. Cozens R. M., Tuomanen E., Tosch W., Zak O., Suter J., Tomasz A. Evaluation of the bactericidal activity of beta-lactam antibiotics on slowly growing bacteria cultured in the chemostat. Antimicrob Agents Chemother. 1986 May;29(5):797–802. doi: 10.1128/aac.29.5.797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Labriola J. D., Mascaro J., Alpert B. The microbiologic flora of orofacial abscesses. J Oral Maxillofac Surg. 1983 Nov;41(11):711–714. doi: 10.1016/0278-2391(83)90186-6. [DOI] [PubMed] [Google Scholar]
  4. Lewis M. A., MacFarlane T. W., McGowan D. A. Quantitative bacteriology of acute dento-alveolar abscesses. J Med Microbiol. 1986 Mar;21(2):101–104. doi: 10.1099/00222615-21-2-101. [DOI] [PubMed] [Google Scholar]
  5. Marsh P. D., Hunter J. R., Bowden G. H., Hamilton I. R., McKee A. S., Hardie J. M., Ellwood D. C. The influence of growth rate and nutrient limitation on the microbial composition and biochemical properties of a mixed culture of oral bacteria grown in a chemostat. J Gen Microbiol. 1983 Mar;129(3):755–770. doi: 10.1099/00221287-129-3-755. [DOI] [PubMed] [Google Scholar]
  6. Moenning J. E., Nelson C. L., Kohler R. B. The microbiology and chemotherapy of odontogenic infections. J Oral Maxillofac Surg. 1989 Sep;47(9):976–985. doi: 10.1016/0278-2391(89)90383-2. [DOI] [PubMed] [Google Scholar]
  7. Rams T. E., Babalola O. O., Slots J. Subgingival occurrence of enteric rods, yeasts and staphylococci after systemic doxycycline therapy. Oral Microbiol Immunol. 1990 Jun;5(3):166–168. doi: 10.1111/j.1399-302x.1990.tb00416.x. [DOI] [PubMed] [Google Scholar]
  8. Slots J., Feik D., Rams T. E. Prevalence and antimicrobial susceptibility of Enterobacteriaceae, Pseudomonadaceae and Acinetobacter in human periodontitis. Oral Microbiol Immunol. 1990 Jun;5(3):149–154. doi: 10.1111/j.1399-302x.1990.tb00413.x. [DOI] [PubMed] [Google Scholar]
  9. Slots J., Rams T. E. Antibiotics in periodontal therapy: advantages and disadvantages. J Clin Periodontol. 1990 Aug;17(7 ):479–493. doi: 10.1111/j.1365-2710.1992.tb01220.x. [DOI] [PubMed] [Google Scholar]
  10. Taylor P. A., leB Williams P. J. Theoretical studies on the coexistence of competing species under continuous-flow conditions. Can J Microbiol. 1975 Jan;21(1):90–98. doi: 10.1139/m75-013. [DOI] [PubMed] [Google Scholar]
  11. Tuomanen E., Cozens R., Tosch W., Zak O., Tomasz A. The rate of killing of Escherichia coli by beta-lactam antibiotics is strictly proportional to the rate of bacterial growth. J Gen Microbiol. 1986 May;132(5):1297–1304. doi: 10.1099/00221287-132-5-1297. [DOI] [PubMed] [Google Scholar]
  12. Vollaard E. J., Clasener H. A., van Saene H. K., Muller N. F. Effect on colonization resistance: an important criterion in selecting antibiotics. DICP. 1990 Jan;24(1):60–66. doi: 10.1177/106002809002400113. [DOI] [PubMed] [Google Scholar]
  13. Williams B. L., McCann G. F., Schoenknecht F. D. Bacteriology of dental abscesses of endodontic origin. J Clin Microbiol. 1983 Oct;18(4):770–774. doi: 10.1128/jcm.18.4.770-774.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wright N. E., Gilbert P. Antimicrobial activity of n-alkyltrimethylammonium bromides: influence of specific growth rate and nutrient limitation. J Pharm Pharmacol. 1987 Sep;39(9):685–690. doi: 10.1111/j.2042-7158.1987.tb06970.x. [DOI] [PubMed] [Google Scholar]
  15. Wright N. E., Gilbert P. Influence of specific growth rate and nutrient-limitation upon the sensitivity of Escherichia coli towards polymyxin B. J Antimicrob Chemother. 1987 Sep;20(3):303–312. doi: 10.1093/jac/20.3.303. [DOI] [PubMed] [Google Scholar]
  16. ter Steeg P. F., Van der Hoeven J. S., de Jong M. H., van Munster P. J., Jansen M. J. Enrichment of subgingival microflora on human serum leading to accumulation of Bacteroides species, Peptostreptococci and Fusobacteria. Antonie Van Leeuwenhoek. 1987;53(4):261–272. doi: 10.1007/BF00393933. [DOI] [PubMed] [Google Scholar]

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