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. 2008 Mar 27;149(7):3512–3520. doi: 10.1210/en.2008-0052

Figure 3.

Figure 3

Effects of forskolin and PMA on CRH hnRNA levels in primary cultures of hypothalamic cells. A, Time course of the effects of forskolin on CRH hnRNA in primary hypothalamic cultures. Bars are the mean and se of the results of four experiments, each performed in duplicate incubations and triplicate qRT-PCR. *, P < 0.01 vs. basal; #, P < 0.01 vs. 0.5 and 1 h. B, Synergistic action of forskolin and PMA on CRH hnRNA production in hypothalamic cell cultures. Cells were incubated for 45 min with increasing concentrations of forskolin in the presence and absence of PMA before measurement of CRH hnRNA using qRT-PCR. Data are the mean and se of values obtained in three to 11 experiments per point. Each experiment was performed in duplicate incubations and triplicate qRT-PCR. *, P < 0.05 vs. basal; **, P < 0.001.