Figure 8.

GnRH and activin modulate Dex-induced human αGSU gene expression synergistically. The 1.8-kb αGSUluc reporter gene was transiently transfected into LβT2 cells with or without overexpression of GR (200 ng). Cells were serum starved overnight and then treated for 24 h with vehicle, 100 nm Dex, 10 nm GnRH, 10 ng/ml activin, 100 nm Dex with 10 nm GnRH, or 100 nm Dex with 10 ng/ml activin. Data represent the mean ± sem of at least three experiments performed in triplicate and are presented as fold induction relative to the control. Daggers (†) represent synergy between hormone treatments by two-way ANOVA, and asterisks (*) represent significant induction by hormone by one-way ANOVA, P < 0.05.