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. 1991 Oct;35(10):2161–2164. doi: 10.1128/aac.35.10.2161

Aminoglycosides modify the in vitro metachromatic reaction and murine generalized Shwartzman phenomenon induced by Salmonella minnesota R595 lipopolysaccharide.

A Focà 1, G Matera 1, D Iannello 1, M C Berlinghieri 1, M C Liberto 1
PMCID: PMC245348  PMID: 1759842

Abstract

Endotoxin-neutralizing activity may be an important property for antibiotics to be used in severe sepsis. Several antibiotics, belonging to different classes, were evaluated as to their endotoxin-neutralizing ability, using the inhibition of an in vitro metachromatic assay for lipopolysaccharides and a murine generalized Shwartzman reaction model. Gentamicin, amikacin, and sisomicin have been found to share significant in vitro antiendotoxin activity at an antibiotic/endotoxin ratio as low as 1.0/5 (by weight) and to reduce the murine generalized Shwartzman reaction at an antibiotic/endotoxin ratio of 3.3/5.

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Selected References

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  1. Artenstein A. W., Cross A. S. Inhibition of endotoxin reactivity by aminoglycosides. J Antimicrob Chemother. 1989 Nov;24(5):826–828. doi: 10.1093/jac/24.5.826-a. [DOI] [PubMed] [Google Scholar]
  2. Billiau A., Heremans H., Vandekerckhove F., Dillen C. Anti-interferon-gamma antibody protects mice against the generalized Shwartzman reaction. Eur J Immunol. 1987 Dec;17(12):1851–1854. doi: 10.1002/eji.1830171228. [DOI] [PubMed] [Google Scholar]
  3. Cohen J., McConnell J. S. Release of endotoxin from bacteria exposed to ciprofloxacin and its prevention with polymyxin B. Eur J Clin Microbiol. 1986 Feb;5(1):13–17. doi: 10.1007/BF02013454. [DOI] [PubMed] [Google Scholar]
  4. Cooperstock M. S. Inactivation of endotoxin by polymyxin B. Antimicrob Agents Chemother. 1974 Oct;6(4):422–425. doi: 10.1128/aac.6.4.422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Corrigan J. J., Jr, Bell B. M. Endotoxin-induced intravascular coagulation: prevention with polymyxin B sulfate. J Lab Clin Med. 1971 May;77(5):802–810. [PubMed] [Google Scholar]
  6. Craig W. A., Turner J. H., Kunin C. M. Prevention of the generalized Shwartzman reaction and endotoxin lethality by polymyxin B localized in tissues. Infect Immun. 1974 Aug;10(2):287–292. doi: 10.1128/iai.10.2.287-292.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Deitch E. A., Ma L., Ma W. J., Grisham M. B., Granger D. N., Specian R. D., Berg R. D. Inhibition of endotoxin-induced bacterial translocation in mice. J Clin Invest. 1989 Jul;84(1):36–42. doi: 10.1172/JCI114164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Flynn P. M., Shenep J. L., Gigliotti F., Davis D. S., Hildner W. K. Immunolabeling of lipopolysaccharide liberated from antibiotic-treated Escherichia coli. Infect Immun. 1988 Oct;56(10):2760–2762. doi: 10.1128/iai.56.10.2760-2762.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hopkin D. A. Frapper fort ou frapper doucement: a gram-negative dilemma. Lancet. 1978 Dec 2;2(8101):1193–1194. [PubMed] [Google Scholar]
  10. Keler T., Nowotny A. Metachromatic assay for the quantitative determination of bacterial endotoxins. Anal Biochem. 1986 Jul;156(1):189–193. doi: 10.1016/0003-2697(86)90172-7. [DOI] [PubMed] [Google Scholar]
  11. Kusser W. C., Ishiguro E. E. Effects of aminoglycosides and spectinomycin on the synthesis and release of lipopolysaccharide by Escherichia coli. Antimicrob Agents Chemother. 1988 Aug;32(8):1247–1250. doi: 10.1128/aac.32.8.1247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moore R. A., Bates N. C., Hancock R. E. Interaction of polycationic antibiotics with Pseudomonas aeruginosa lipopolysaccharide and lipid A studied by using dansyl-polymyxin. Antimicrob Agents Chemother. 1986 Mar;29(3):496–500. doi: 10.1128/aac.29.3.496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morrison D. C., Jacobs D. M. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry. 1976 Oct;13(10):813–818. doi: 10.1016/0019-2791(76)90181-6. [DOI] [PubMed] [Google Scholar]
  14. Novitsky T. J., Roslansky P. F., Siber G. R., Warren H. S. Turbidimetric method for quantifying serum inhibition of Limulus amoebocyte lysate. J Clin Microbiol. 1985 Feb;21(2):211–216. doi: 10.1128/jcm.21.2.211-216.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Peterson A. A., Fesik S. W., McGroarty E. J. Decreased binding of antibiotics to lipopolysaccharides from polymyxin-resistant strains of Escherichia coli and Salmonella typhimurium. Antimicrob Agents Chemother. 1987 Feb;31(2):230–237. doi: 10.1128/aac.31.2.230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Raetz C. R. Biochemistry of endotoxins. Annu Rev Biochem. 1990;59:129–170. doi: 10.1146/annurev.bi.59.070190.001021. [DOI] [PubMed] [Google Scholar]
  17. Shenep J. L., Flynn P. M., Barrett F. F., Stidham G. L., Westenkirchner D. F. Serial quantitation of endotoxemia and bacteremia during therapy for gram-negative bacterial sepsis. J Infect Dis. 1988 Mar;157(3):565–568. doi: 10.1093/infdis/157.3.565. [DOI] [PubMed] [Google Scholar]
  18. Shenep J. L., Mogan K. A. Kinetics of endotoxin release during antibiotic therapy for experimental gram-negative bacterial sepsis. J Infect Dis. 1984 Sep;150(3):380–388. doi: 10.1093/infdis/150.3.380. [DOI] [PubMed] [Google Scholar]

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