FIG. 2.

PPLNs contain T-cells reactive with PI-1(47–64). A: T-cells from the PPLNs of NOD mice from 10–16 weeks of age contain PI-1(47–64)–reactive T-cells. Five of 246 expanded T-cell lines from the PPLNs reacted with the SPGDLQTLALEVARQKRG peptide with a stimulation index (SI) >5. In contrast, 0 of 454 T-cell lines expanded from the spleen or axillary and inguinal lymph nodes were stimulated by the PI-1(47–64) peptide. B: Data from one representative experiment in which 41 T-cell lines from the PPLNs were tested for reactivity with the PI-1(47–64) peptide.