TABLE 2.
Binding to I-Ag7
| Peptide | Sequence | Binding |
|
|---|---|---|---|
| I-Ag7 | I-Ak | ||
| MIME | GKKVATTVHAGYG | 1 | — |
| PI-1(47–64) | SPGDLQTLALEVARQKRG | 98.9 | 4.8 |
| PI-2(49–66) | GAGDLQTLALEVAQQKRG | 135.9 | 2.1 |
| PI-1(49–61) | GDLQTLALEVARQ | 20.0 | — |
| INS-B(9–23) | SHLVEALYLVCGERG | 3 | — |
This panel shows the binding analysis of synthetic peptides to soluble I-Ag7 and I-Ak. Binding is expressed by IC50 values (μmol/l). The mime peptide is a synthetic reference peptide that has been used in I-Ag7 binding assays (16). The binding of mouse insulin-2 B:(9–23) (3 μmol/l) peptide is presented as a point of reference. Binding studies were performed at pH 5.5 as described in research design and methods.