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. 1991 Nov;35(11):2359–2365. doi: 10.1128/aac.35.11.2359

Induction of a class I beta-lactamase from Citrobacter freundii in Escherichia coli requires active ftsZ but not ftsA or ftsQ products.

A C Ottolenghi 1, J A Ayala 1
PMCID: PMC245385  PMID: 1804009

Abstract

A possible connection between septation/division and induction of cloned ampC beta-lactamase was investigated. When a ftsZ84(Ts) mutant of Escherichia coli carrying ampR-ampC from Citrobacter freundii was grown at the restrictive temperature (42 degrees C), induction of beta-lactamase by cefoxitin was inhibited by about 80%. Inhibition was virtually complete when a ftsZ84(Ts) mutant of different genetic background was tested. Although somewhat delayed, the induction of beta-lactamase in transformed ftsA(Ts) and ftsQ(Ts) mutants was similar to that observed in wild-type transformants. These results imply that FtsZ is involved in the process of beta-lactamase induction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Begg K. J., Donachie W. D. Cell shape and division in Escherichia coli: experiments with shape and division mutants. J Bacteriol. 1985 Aug;163(2):615–622. doi: 10.1128/jb.163.2.615-622.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  3. Cullmann W., Dalhoff A., Dick W. Nonspecific induction of beta-lactamase in Enterobacter cloacae. J Gen Microbiol. 1984 Jul;130(7):1781–1786. doi: 10.1099/00221287-130-7-1781. [DOI] [PubMed] [Google Scholar]
  4. García del Portillo F., de Pedro M. A. Differential effect of mutational impairment of penicillin-binding proteins 1A and 1B on Escherichia coli strains harboring thermosensitive mutations in the cell division genes ftsA, ftsQ, ftsZ, and pbpB. J Bacteriol. 1990 Oct;172(10):5863–5870. doi: 10.1128/jb.172.10.5863-5870.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gatus B. J., Bell S. M., Jimenez A. S. Comparison of glycine enhancement with cefoxitin induction of class 1 beta-lactamase production in Enterobacter cloacae ATCC 13047. J Antimicrob Chemother. 1988 Feb;21(2):163–170. doi: 10.1093/jac/21.2.163. [DOI] [PubMed] [Google Scholar]
  6. Gatus B. J., Bell S. M., Jimenez A. S. Enhancement of beta-lactamase production by glycine in Enterobacter cloacae ATCC.13047. Pathology. 1986 Jan;18(1):145–147. doi: 10.3109/00313028609090843. [DOI] [PubMed] [Google Scholar]
  7. Gatus B. J., Bell S. M., Jimenez A. S. The effect of casein derivatives on glycine enhancement of beta-lactamase production in Enterobacter cloacae ATCC.13047. Pathology. 1986 Oct;18(4):379–381. doi: 10.3109/00313028609087554. [DOI] [PubMed] [Google Scholar]
  8. Hammes W., Schleifer K. H., Kandler O. Mode of action of glycine on the biosynthesis of peptidoglycan. J Bacteriol. 1973 Nov;116(2):1029–1053. doi: 10.1128/jb.116.2.1029-1053.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Korfmann G., Sanders C. C., Moland E. S. Altered phenotypes associated with ampD mutations in Enterobacter cloacae. Antimicrob Agents Chemother. 1991 Feb;35(2):358–364. doi: 10.1128/aac.35.2.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Korfmann G., Sanders C. C. ampG is essential for high-level expression of AmpC beta-lactamase in Enterobacter cloacae. Antimicrob Agents Chemother. 1989 Nov;33(11):1946–1951. doi: 10.1128/aac.33.11.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  12. Lindberg F., Lindquist S., Normark S. Inactivation of the ampD gene causes semiconstitutive overproduction of the inducible Citrobacter freundii beta-lactamase. J Bacteriol. 1987 May;169(5):1923–1928. doi: 10.1128/jb.169.5.1923-1928.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lindberg F., Westman L., Normark S. Regulatory components in Citrobacter freundii ampC beta-lactamase induction. Proc Natl Acad Sci U S A. 1985 Jul;82(14):4620–4624. doi: 10.1073/pnas.82.14.4620. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lindquist S., Galleni M., Lindberg F., Normark S. Signalling proteins in enterobacterial AmpC beta-lactamase regulation. Mol Microbiol. 1989 Aug;3(8):1091–1102. doi: 10.1111/j.1365-2958.1989.tb00259.x. [DOI] [PubMed] [Google Scholar]
  15. Lutkenhaus J. F. Coupling of DNA replication and cell division: sulB is an allele of ftsZ. J Bacteriol. 1983 Jun;154(3):1339–1346. doi: 10.1128/jb.154.3.1339-1346.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oliva B., Bennett P. M., Chopra I. Penicillin-binding protein 2 is required for induction of the Citrobacter freundii class I chromosomal beta-lactamase in Escherichia coli. Antimicrob Agents Chemother. 1989 Jul;33(7):1116–1117. doi: 10.1128/aac.33.7.1116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Prats R., Gomez M., Pla J., Blasco B., Ayala J. A. A new beta-lactam-binding protein derived from penicillin-binding protein 3 of Escherichia coli. J Bacteriol. 1989 Sep;171(9):5194–5198. doi: 10.1128/jb.171.9.5194-5198.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Prats R., de Pedro M. A. Normal growth and division of Escherichia coli with a reduced amount of murein. J Bacteriol. 1989 Jul;171(7):3740–3745. doi: 10.1128/jb.171.7.3740-3745.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sanders C. C. Chromosomal cephalosporinases responsible for multiple resistance to newer beta-lactam antibiotics. Annu Rev Microbiol. 1987;41:573–593. doi: 10.1146/annurev.mi.41.100187.003041. [DOI] [PubMed] [Google Scholar]
  20. Tuomanen E., Cozens R. Changes in peptidoglycan composition and penicillin-binding proteins in slowly growing Escherichia coli. J Bacteriol. 1987 Nov;169(11):5308–5310. doi: 10.1128/jb.169.11.5308-5310.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tuomanen E., Lindquist S., Sande S., Galleni M., Light K., Gage D., Normark S. Coordinate regulation of beta-lactamase induction and peptidoglycan composition by the amp operon. Science. 1991 Jan 11;251(4990):201–204. doi: 10.1126/science.1987637. [DOI] [PubMed] [Google Scholar]

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