Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Jul;119(1):44–49. doi: 10.1128/jb.119.1.44-49.1974

Changes in Microsporum gypseum Mycelial Wall and Spore Coat Glycoproteins During Sporulation and Spore Germination

W J Page a,1, J J Stock a
PMCID: PMC245570  PMID: 4407013

Abstract

Ethylenediamine-soluble glycoproteins were extracted from isolated Microsporum gypseum hyphal walls during sporulation and from spore coats before and after germination. This study was carried out to identify a sporulation-specific cell wall protein that possibly served as a substrate for the alkaline protease which initiated the macroconidial germination of this fungus. Analyses revealed that water-insoluble glycoprotein accounted for 10% of the ungerminated spore coat but only for 4 to 5% of the mycelial wall dry weight. This fraction was modified in its amino acid composition during sporulation, and it decreased in protein content during spore germination. Water-soluble glycoprotein, which accounted for approximately 3 to 3.5% of either the spore coat or mycelial wall dry weight, was of similar amino acid composition from both sources and did not decrease in protein content upon spore germination. The water-insoluble glycoprotein was found to be rich in leucine, aspartic acid, glycine, glutamic acid, and phenylalanine residues. The water-soluble glycoprotein was rich in proline, threonine, glycine, serine, glutamic acid, and alanine.

Full text

PDF
44

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTNICKI-GARCIA S., NICKERSON W. J. Isolation, composition, and structure of cell walls of filamentous and yeast-like forms of Mucor rouxii. Biochim Biophys Acta. 1962 Mar 26;58:102–119. doi: 10.1016/0006-3002(62)90822-3. [DOI] [PubMed] [Google Scholar]
  2. Bartnicki-Garcia S. Cell wall chemistry, morphogenesis, and taxonomy of fungi. Annu Rev Microbiol. 1968;22:87–108. doi: 10.1146/annurev.mi.22.100168.000511. [DOI] [PubMed] [Google Scholar]
  3. Bleyman M., Woese C. Ribosomal ribonucleic acid maturation during bacterial spore germination. J Bacteriol. 1969 Jan;97(1):27–31. doi: 10.1128/jb.97.1.27-31.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dulaney J. T., Touster O. The solubilization and gel electrophoresis of membrane enzymes by use of detergents. Biochim Biophys Acta. 1970 Jan 6;196(1):29–34. doi: 10.1016/0005-2736(70)90162-8. [DOI] [PubMed] [Google Scholar]
  5. Fisher D. J., Richmond D. V. The electrophoretic properties and some surface components of penicillium conidia. J Gen Microbiol. 1970 Dec;64(2):205–214. doi: 10.1099/00221287-64-2-205. [DOI] [PubMed] [Google Scholar]
  6. Grappel S. F., Blank F., Bishop C. T. Immunological studies on dermatophytes. IV. Chemical structures and serological reactivities of polysaccharides from Microsporum praecox, Trichophyton ferrugineum, Trichophyton sabouraudii, and Trichophyton tonsurans. J Bacteriol. 1969 Jan;97(1):23–26. doi: 10.1128/jb.97.1.23-26.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KORN E. D., NORTHCOTE D. H. Physical and chemical properties of polysaccharides and glycoproteins of the yeast-cell wall. Biochem J. 1960 Apr;75:12–17. doi: 10.1042/bj0750012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Leighton T. J., Stock J. J. Biochemical changes during fungal sporulation and spore germination. I. Phenyl methyl sulfonyl fluoride inhibition of macroconidial germination in Microsporum gypseum. J Bacteriol. 1970 Mar;101(3):931–940. doi: 10.1128/jb.101.3.931-940.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leighton T. J., Stock J. J. Heat-induced macroconidia germination in Microsporum gypseum. Appl Microbiol. 1969 Mar;17(3):473–475. doi: 10.1128/am.17.3.473-475.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leighton T. J., Stock J. J. Isolation and preliminary characterization of developmental mutants from Microsporum gypseum. J Bacteriol. 1970 Nov;104(2):834–838. doi: 10.1128/jb.104.2.834-838.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MCDONALD C. E., CHEN L. L. THE LOWRY MODIFICATION OF THE FOLIN REAGENT FOR DETERMINATION OF PROTEINASE ACTIVITY. Anal Biochem. 1965 Jan;10:175–177. doi: 10.1016/0003-2697(65)90255-1. [DOI] [PubMed] [Google Scholar]
  13. Morris D. L. Quantitative Determination of Carbohydrates With Dreywood's Anthrone Reagent. Science. 1948 Mar 5;107(2775):254–255. doi: 10.1126/science.107.2775.254. [DOI] [PubMed] [Google Scholar]
  14. NICKERSON W. J. SYMPOSIUM ON BIOCHEMICAL BASES OF MORPHOGENESIS IN FUNGI. IV. MOLECULAR BASES OF FORM IN YEASTS. Bacteriol Rev. 1963 Sep;27:305–324. doi: 10.1128/br.27.3.305-324.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Page W. J., Stock J. J. Initiation of dermatophyte pleomorphic strain sporulation by increased aeration. Appl Microbiol. 1972 Oct;24(4):650–657. doi: 10.1128/am.24.4.650-657.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Page W. J., Stock J. J. Isolation and characterization of Microsporum gypseum lysosomes: role of lysosomes in macroconidia germination. J Bacteriol. 1972 Apr;110(1):354–362. doi: 10.1128/jb.110.1.354-362.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Page W. J., Stock J. J. Regulation and self-inhibition of Microsporum gypseum Macroconidia germination. J Bacteriol. 1971 Oct;108(1):276–281. doi: 10.1128/jb.108.1.276-281.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Warth A. D., Strominger J. L. Structure of the peptidoglycan from vegetative cell walls of Bacillus subtilis. Biochemistry. 1971 Nov 23;10(24):4349–4358. doi: 10.1021/bi00800a001. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES