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. 1974 Jul;119(1):138–143. doi: 10.1128/jb.119.1.138-143.1974

Transport of d-Arabinose-5-Phosphate and d-Sedoheptulose-7-Phosphate by the Hexose Phosphate Transport System of Salmonella typhimurium

Leon Eidels a,1, Paul D Rick a, Norma P Stimler a, M J Osborn a
PMCID: PMC245583  PMID: 4600697

Abstract

d-Arabinose-5-phosphate and d-sedoheptulose-7-phosphate were found to be substrates, although not inducers, of the hexose phosphate transport system of Salmonella typhimurium. Transport of these two sugar phosphates by wild-type strains required preinduction of the hexose phosphate transport system. A mutant of S. typhimurium constitutive for this system also transported d-arabinose-5-phosphate and d-sedoheptulose-7-phosphate in a constitutive fashion. Glucose-6-phosphate was a potent competitor of the transport of both d-arabinose-5-phosphate and d-sedoheptulose-7-phosphate. The Km values for transport of d-glucose-6-phosphate, d-arabinose-5-phosphate, and d-sedoheptulose-7-phosphate were 0.13, 0.32 and 1.61 mM, respectively. The apparent Vmax values for transport of d-glucose-6-phosphate, d-arabinose-5-phosphate, and d-sedoheptulose-7-phosphate were 6.3, 13.2 and 3.0 nmol per min per 5 × 108 bacteria, respectively. d-Ribulose-5-phosphate and d-xylulose-5-phosphate did not inhibit transport of the above substrates, whereas d-ribose-5-phosphate was a weak inhibitor of d-sedoheptulose-7-phosphate transport.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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