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. 1974 Aug;119(2):339–344. doi: 10.1128/jb.119.2.339-344.1974

Halophilic Nuclease from a Moderately Halophilic Micrococcus varians

Masahiro Kamekura a, Hiroshi Onishi a,1
PMCID: PMC245612  PMID: 4852218

Abstract

The moderately halophilic bacterium Micrococcus varians, isolated from soy sauce mash, produced extracellular nuclease when cultivated aerobically in media containing 1 to 4 M NaCl or KCl. The enzyme, purified to an electrophoretically homogeneous state, had both ribonuclease and deoxyribonuclease activities. The nuclease had maximal activity in the presence of 2.9 M NaCl or 2.1 M KCl at 40 C. The enzymatic activity was lost by dialysis against low-salt buffer, whereas when the inactivated enzyme was dialyzed against 3.4 M NaCl buffer as much as 77% of the initial activity could be restored.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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