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. 1974 Aug;119(2):386–393. doi: 10.1128/jb.119.2.386-393.1974

Mechanism of α Factor Biosynthesis in Saccharomyces cerevisiae

Günther Scherer 1, Georg Haag 1, Wolfgang Duntze 1
PMCID: PMC245619  PMID: 4604734

Abstract

The biosynthesis of α factor, a mating-type-specific regulatory oligopeptide which is secreted by Saccharomyces cerevisiae cells of α mating type, was studied. In batch cultures only small amounts of the peptide were synthesized during the exponential growth phase. During the stationary phase, α factor was produced at a constant rate and accumulated in the culture medium. Inhibition of translation in wild-type cells by cycloheximide, or in mutant strains under conditions which blocked protein or ribonucleic acid (RNA) synthesis completely inhibited the production of α factor. These results indicate that the factor is produced by ribosomal translation of a specific messenger RNA and not by an extraribosomal mechanism of peptide synthesis.

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Selected References

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