Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Aug;119(2):478–483. doi: 10.1128/jb.119.2.478-483.1974

Incompatibility Exhibited by Colicin Plasmids E1, E2, and E3 in Escherichia coli

Joseph Inselburg 1
PMCID: PMC245630  PMID: 4604597

Abstract

Escherichia coli strains were made multiply colicinogenic for the colicin plasmids E1, E2, or E3 (Col E1, Col E2, or Col E3, respectively) by both a deoxyribonucleic acid transformation system and bacterial conjugation. The multiply colicinogenic bacteria constructed exhibited an immunity to the colicins produced by all the plasmids they carried and also produced colicins corresponding to all the plasmids they carried. An incompatibility was observed among the plasmids. In doubly colicinogenic cells where the presence of two plasmids was established, Col E2 was lost more frequently than Col E3. In triply colicinogenic cells, Col E1, Col E2, and Col E3 were lost, with Col E3 being lost least frequently. A significant reduction in the acquisition of a conjugationally transferred Col E1 plasmid by cells colicinogenic for Col E1 was demonstrated.

Full text

PDF
478

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Almendinger R., Hager L. P. Role for endonuclease I in the transmission process of colicin E 2 . Nat New Biol. 1972 Feb 16;235(59):199–203. doi: 10.1038/newbio235199a0. [DOI] [PubMed] [Google Scholar]
  3. Bazaral M., Helinski D. R. Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. J Mol Biol. 1968 Sep 14;36(2):185–194. doi: 10.1016/0022-2836(68)90374-4. [DOI] [PubMed] [Google Scholar]
  4. Boon T. Inactivation of ribosomes in vitro by colicin E 3 . Proc Natl Acad Sci U S A. 1971 Oct;68(10):2421–2425. doi: 10.1073/pnas.68.10.2421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bowman C. M., Dahlberg J. E., Ikemura T., Konisky J., Nomura M. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo. Proc Natl Acad Sci U S A. 1971 May;68(5):964–968. doi: 10.1073/pnas.68.5.964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cosloy S. D., Oishi M. Genetic transformation in Escherichia coli K12. Proc Natl Acad Sci U S A. 1973 Jan;70(1):84–87. doi: 10.1073/pnas.70.1.84. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dubnau E., Maas W. K. Inhibition of replication of an F'lac episome in Hfr cells of Escherichia coli. J Bacteriol. 1968 Feb;95(2):531–539. doi: 10.1128/jb.95.2.531-539.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FREDERICQ P. Spectres d'activité differents des souches productrices de colicine E ou K et des bactériophages des groupes II ou III. C R Seances Soc Biol Fil. 1951 Sep;145(17-18):1433–1436. [PubMed] [Google Scholar]
  11. Herschman H. R., Helinski D. R. Purification and characterization of colicin E2 and colicin E3. J Biol Chem. 1967 Nov 25;242(22):5360–5368. [PubMed] [Google Scholar]
  12. Inselburg J. Colicin factor DNA: a single non-homologous region in Col E2-E3 heteroduplex molecules. Nat New Biol. 1973 Feb 21;241(112):234–237. doi: 10.1038/newbio241234a0. [DOI] [PubMed] [Google Scholar]
  13. Inselburg J. Isolation and characterization of mutants of colicin plasmids E1 and E2 after Mu bacteriophage infection. J Bacteriol. 1974 Aug;119(2):469–477. doi: 10.1128/jb.119.2.469-477.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kingsbury D. T., Helinski D. R. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538–1544. doi: 10.1016/0006-291x(70)90562-0. [DOI] [PubMed] [Google Scholar]
  15. Kingsbury D. T., Helinski D. R. Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 . J Bacteriol. 1973 Jun;114(3):1116–1124. doi: 10.1128/jb.114.3.1116-1124.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Krcméry V., Hurwitz C., Fredericq P. Loss of colicinogeny in Escherichia coli strains infected by certain resistance factors. J Bacteriol. 1970 May;102(2):521–523. doi: 10.1128/jb.102.2.521-523.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LURIA S. E. ON THE MECHANISMS OF ACTION OF COLICINS. Ann Inst Pasteur (Paris) 1964 Nov;107:SUPPL–SUPPL:73. [PubMed] [Google Scholar]
  18. LeBlanc D. J., Falkow S. Studies on superinfection immunity among transmissible plasmids in Escherichia coli. J Mol Biol. 1973 Mar 15;74(4):689–701. doi: 10.1016/0022-2836(73)90057-0. [DOI] [PubMed] [Google Scholar]
  19. Mandel M., Higa A. Calcium-dependent bacteriophage DNA infection. J Mol Biol. 1970 Oct 14;53(1):159–162. doi: 10.1016/0022-2836(70)90051-3. [DOI] [PubMed] [Google Scholar]
  20. NOMURA M. MECHANISM OF ACTION OF COLICINES. Proc Natl Acad Sci U S A. 1964 Dec;52:1514–1521. doi: 10.1073/pnas.52.6.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Novick R. P. Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969 Jun;33(2):210–263. doi: 10.1128/br.33.2.210-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. OZEKI H., STOCKER B. A., DE MARGERIE H. Production of colicine by single bacteria. Nature. 1959 Aug 1;184:337–339. doi: 10.1038/184337a0. [DOI] [PubMed] [Google Scholar]
  23. Reeves P. Mutants resistant to colicin CA42-E2: cross resistance and genetic mapping of a special class of mutants. Aust J Exp Biol Med Sci. 1966 Jun;44(3):301–315. doi: 10.1038/icb.1966.29. [DOI] [PubMed] [Google Scholar]
  24. SCAIFE J., GROSS J. D. Inhibition of multiplication of an Flac factor in Hfr cells of Escherichia coli K-12. Biochem Biophys Res Commun. 1962 May 11;7:403–407. doi: 10.1016/0006-291x(62)90324-8. [DOI] [PubMed] [Google Scholar]
  25. Schwartz S. A., Helinski D. R. Purification and characterization of colicin E1. J Biol Chem. 1971 Oct 25;246(20):6318–6327. [PubMed] [Google Scholar]
  26. Sherratt D. J., Helinski D. R. Replication of colicinogenic factor E1 in Escherichia coli. Properties of newly replicated supercoils. Eur J Biochem. 1973 Aug 1;37(1):95–99. doi: 10.1111/j.1432-1033.1973.tb02962.x. [DOI] [PubMed] [Google Scholar]
  27. Shull F. W., Jr, Fralick J. A., Stratton L. P., Fisher W. D. Membrane association of conjugally transferred deoxyribonucleic acid in Escherichia coli minicells. J Bacteriol. 1971 May;106(2):626–633. doi: 10.1128/jb.106.2.626-633.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sidikaro J., Nomura M. E3 immunity substance. A protein from e3-colicinogenic cells that accounts for their immunity to colicin E3. J Biol Chem. 1974 Jan 25;249(2):445–453. [PubMed] [Google Scholar]
  29. Strobel M., Nomura M. Restriction of the growth of bacteriophage BF23 by a colicine I (Col I-P9) factor. Virology. 1966 Apr;28(4):763–765. doi: 10.1016/0042-6822(66)90263-7. [DOI] [PubMed] [Google Scholar]
  30. WATANABE T., FUKASAWA T. Episome-mediated transfer of drug resistance in Enterobacteriaceae IV. Interactions between resistance transfer factor and F-factor in Escherichia coli K-12. J Bacteriol. 1962 Apr;83:727–735. doi: 10.1128/jb.83.4.727-735.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES