Abstract
Lipid analyses of the anaerobic bacterium Sphaerophorus ridiculosis revealed that 24.2% of the polar lipids are the alk-1′-enyl glyceryl ether (plasmalogen) form. The major polar lipids, phosphatidylethanolamine (67.5%), phosphatidylglycerol (11.2%), cardiolipin (12.0%), and lyso-phosphatidylethanolamine (9.3%), contained 26.3, 7.8, 5.2, and 13.4% plasmalogen, respectively.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
- BAUMANN N. A., HAGEN P. O., GOLDFINE H. PHOSPHOLIPIDS OF CLOSTRIDIUM BUTYRICUM. STUDIES ON PLASMALOGEN COMPOSITION AND BIOSYNTHESIS. J Biol Chem. 1965 Apr;240:1559–1567. [PubMed] [Google Scholar]
- DAWSON R. M. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. doi: 10.1042/bj0750045. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
- Goldfine H., Hagen P. N-methyl groups in bacterial lipids. 3. Phospholipids of hyphomicrobia. J Bacteriol. 1968 Feb;95(2):367–375. doi: 10.1128/jb.95.2.367-375.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HANES C. S., ISHERWOOD F. A. Separation of the phosphoric esters on the filter paper chromatogram. Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0. [DOI] [PubMed] [Google Scholar]
- Hagen P. O., Goldfine H. Phospholipids of Clostridium butyricum. 3. Further studies on the origin of the aldehyde chains of plasmalogens. J Biol Chem. 1967 Dec 25;242(24):5700–5708. [PubMed] [Google Scholar]
- Kates M., Wassef M. K. Lipid chemistry. Annu Rev Biochem. 1970;39:323–358. doi: 10.1146/annurev.bi.39.070170.001543. [DOI] [PubMed] [Google Scholar]
- Meyer H., Meyer F. Lipid metabolism in the parasitic and free-living spirochetes Treponema pallidum (Reiter) and Treponema zuelzerae. Biochim Biophys Acta. 1971 Feb 2;231(1):93–106. doi: 10.1016/0005-2760(71)90257-8. [DOI] [PubMed] [Google Scholar]
- PRIVETT O. S., BLANK M. L., CODDING D. W., NICKELL E. C. LIPID ANALYSIS BY QUANTITATIVE THIN-LAYER CHROMATOGRAPHY. J Am Oil Chem Soc. 1965 May;42:381–393. doi: 10.1007/BF02635573. [DOI] [PubMed] [Google Scholar]
- Pearson T. A., Balish E. Unusual Sphaerophorus species from the large intestine of man. Appl Microbiol. 1970 Mar;19(3):458–462. doi: 10.1128/am.19.3.458-462.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Snyder F., Kimble H. An automatic zonal scraper and sample collector for radioassay of thin-layer chromatograms. Anal Biochem. 1965 Jun;11(3):510–518. doi: 10.1016/0003-2697(65)90069-2. [DOI] [PubMed] [Google Scholar]
- Vandenheuvel W. J., Gardiner W. L., Horning E. C. Gas chromatographic behavior of long chain alcohols and their derivatives. J Chromatogr. 1965 Aug;19(2):263–276. doi: 10.1016/s0021-9673(01)99459-2. [DOI] [PubMed] [Google Scholar]
- Waku K., Uda Y., Nakazawa Y. Lipid composition in rabbit sarcoplasmic reticulum and occurrence of alkyl ether phospholipids. J Biochem. 1971 Mar;69(3):483–491. [PubMed] [Google Scholar]
- Wood R., Snyder F. Chemical and physical properties of isomeric glyceryl monoethers. Lipids. 1967 Mar;2(2):161–171. doi: 10.1007/BF02530917. [DOI] [PubMed] [Google Scholar]
- Wood R., Snyder F. Quantitative determination of alk-1-enyl- and alkyl-glyceryl ethers in neutral lipids and phospholipids. Lipids. 1968 Mar;3(2):129–135. doi: 10.1007/BF02531729. [DOI] [PubMed] [Google Scholar]