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. 1974 Sep;119(3):661–665. doi: 10.1128/jb.119.3.661-665.1974

Citrate Metabolism in Aerobacter cloacae

R W O'Brien 1, Jarmila Geisler 1
PMCID: PMC245665  PMID: 4211873

Abstract

Growth of Aerobacter cloacae on citrate either anaerobically or aerobically did not require and was not stimulated by the presence of Na+ in the medium. Citrate was metabolized anaerobically via the fermentation pathway as evidenced by the (i) presence of oxalacetate decarboxylase, (ii) induction of citrate lyase, and (iii) repression of α-ketoglutarate dehydrogenase under anaerobic conditions. Thus, although all the other enzymes of the citric acid cycle were present in anaerobic cells, this pathway was not available for the metabolism of citrate. Citrate was metabolized aerobically via the citric acid cycle, since (i) citrate lyase but not oxalacetate decarboxylase was repressed and (ii) α-ketoglutarate dehydrogenase was induced under these conditions. The presence of Na+ in the medium did not lead to a repression of α-ketoglutarate dehydrogenase as in the case of Aerobacter aerogenes. The oxalacetate decarboxylase was a soluble, constitutive enzyme, not activated by Na+ nor inhibited by avidin. It was slightly inhibited by ethylenediaminetetraacetate but was not stimulated by Mg2+ or Mn2+. Thus, this enzyme differed markedly in its properties from the same enzyme found in citrate-grown A. aerogenes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amarasingham C. R., Davis B. D. Regulation of alpha-ketoglutarate dehydrogenase formation in Escherichia coli. J Biol Chem. 1965 Sep;240(9):3664–3668. [PubMed] [Google Scholar]
  2. BENZIMAN M., HELLER N. OXALOACETATE DECARBOXYLATION AND OXALOACETATE-CARBON DIOXIDE EXCHANGE IN ACETOBACTER XYLINUM. J Bacteriol. 1964 Dec;88:1678–1687. doi: 10.1128/jb.88.6.1678-1687.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DAGLEY S., DAWES E. A. Dissimilation of citric acid by bacterial extracts. Nature. 1953 Aug 15;172(4372):345–346. doi: 10.1038/172345a0. [DOI] [PubMed] [Google Scholar]
  4. Eagon R. G., Wilkerson L. S. A potassium-dependent citric acid transport system in Aerobacter aerogenes. Biochem Biophys Res Commun. 1972 Mar 10;46(5):1944–1950. doi: 10.1016/0006-291x(72)90074-5. [DOI] [PubMed] [Google Scholar]
  5. FRANCIS M. J., HUGHES D. E., KORNBERG H. L., PHIZACKERLEY P. J. THE OXIDATION OF L-MALATE BY PSEUDOMONAS SP. Biochem J. 1963 Dec;89:430–438. doi: 10.1042/bj0890430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gray C. T., Wimpenny J. W., Mossman M. R. Regulation of metabolism in facultative bacteria. II. Effects of aerobiosis, anaerobiosis and nutrition on the formation of Krebs cycle enzymes in Escherichia coli. Biochim Biophys Acta. 1966 Mar 28;117(1):33–41. doi: 10.1016/0304-4165(66)90149-8. [DOI] [PubMed] [Google Scholar]
  7. HORTON A. A., KORNBERG H. L. OXALOACETATE 4-CARBOXY-LYASE FROM PSEUDOMONAS OVALIS CHESTER. Biochim Biophys Acta. 1964 Aug 26;89:381–383. doi: 10.1016/0926-6569(64)90236-6. [DOI] [PubMed] [Google Scholar]
  8. Krampitz L. O., Werkman C. H. The enzymic decarboxylation of oxaloacetate. Biochem J. 1941 Jun;35(5-6):595–602. doi: 10.1042/bj0350595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. O'Brien R. W., Frost G. M., Stern J. R. Enzymatic analysis of the requirement for sodium in aerobic growth of Salmonella typhimurium on citrate. J Bacteriol. 1969 Aug;99(2):395–400. doi: 10.1128/jb.99.2.395-400.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. O'Brien R. W., Stern J. R. Requirement for sodium in the anaerobic growth of Aerobacter aerogenes on citrate. J Bacteriol. 1969 May;98(2):388–393. doi: 10.1128/jb.98.2.388-393.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Brien R. W., Stern J. R. Role of sodium in determining alternate pathways of aerobic citrate catabolism in Aerobacter aerogenes. J Bacteriol. 1969 Aug;99(2):389–394. doi: 10.1128/jb.99.2.389-394.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Rosenberger R. F. Derepression of oxaloacetate 4-carboxy-lyase synthesis in Salmonella typhimurium. Biochim Biophys Acta. 1966 Aug 10;122(2):365–367. doi: 10.1016/0926-6593(66)90079-8. [DOI] [PubMed] [Google Scholar]
  13. SINGER T. P., KEARNEY E. B. Determination of succinic dehydrogenase activity. Methods Biochem Anal. 1957;4:307–333. doi: 10.1002/9780470110201.ch9. [DOI] [PubMed] [Google Scholar]
  14. Stern J. R. Oxalacetate decarboxylase of Aerobacter aerogenes. I. Inhibition by avidin and requirement for sodium ion. Biochemistry. 1967 Nov;6(11):3545–3551. doi: 10.1021/bi00863a028. [DOI] [PubMed] [Google Scholar]
  15. Villarreal-Moguel E. I., Ruiz-Herrera J. Induction and properties of the citrate transport system in Aerobacter aerogenes. J Bacteriol. 1969 May;98(2):552–558. doi: 10.1128/jb.98.2.552-558.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wilkerson L. S., Eagon R. G. Transport of citric acid by Aerobacter aerogenes. Arch Biochem Biophys. 1972 Mar;149(1):209–221. doi: 10.1016/0003-9861(72)90316-5. [DOI] [PubMed] [Google Scholar]

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