Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Sep;119(3):684–690. doi: 10.1128/jb.119.3.684-690.1974

Statistical Estimate of the Total Number of Operons Specific for Bacillus subtilis Sporulation

D Hranueli a,1, P J Piggot a,2, J Mandelstam a
PMCID: PMC245669  PMID: 4212352

Abstract

As an alternative to exhaustive mapping, an attempt has been made to obtain a rough estimate of the total number of sporulation operons by statistical treatment. Sixteen sporulation mutants taken at random were characterized biochemically and morphologically. The mutations they contained were mapped to determine whether they fell into any one of 23 known operons. From the proportion that do so (10/16), it is calculated that the most probable number of sporulation operons is 37 (68% confidence limits of 31 and 46). If allowance is made for the fact that two of the operons apparently contain mutagenic “hot spots” and the calculation is amended accordingly, the most probable numbers of operons becomes 42 (limits 33 and 59).

Full text

PDF
684

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balassa G. The genetic control of spore formation in bacilli. Curr Top Microbiol Immunol. 1971;56:99–192. doi: 10.1007/978-3-642-65241-7_4. [DOI] [PubMed] [Google Scholar]
  2. Carlton B. C. Fine-structure mapping by transformation in the tryptophan region of Bacillus subtilis. J Bacteriol. 1966 May;91(5):1795–1803. doi: 10.1128/jb.91.5.1795-1803.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coote J. G., Mandelstam J. Use of constructed double mutants for determining the temporal order of expression of sporulation genes in Bacillus subtilis. J Bacteriol. 1973 Jun;114(3):1254–1263. doi: 10.1128/jb.114.3.1254-1263.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coote J. G. Sporulation in Bacillus subtilis. Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants. J Gen Microbiol. 1972 Jun;71(1):1–15. doi: 10.1099/00221287-71-1-1. [DOI] [PubMed] [Google Scholar]
  5. Coote J. G. Sporulation in Bacillus subtilis. Genetic analysis of oligosporogenous mutants. J Gen Microbiol. 1972 Jun;71(1):17–27. doi: 10.1099/00221287-71-1-17. [DOI] [PubMed] [Google Scholar]
  6. Ephrati-Elizur E. Spontaneous transformation in Bacillus subtilis. Genet Res. 1968 Feb;11(1):83–96. doi: 10.1017/s0016672300011216. [DOI] [PubMed] [Google Scholar]
  7. Hoch J. A. Genetic analysis of pleiotropic negative sporulation mutants in Bacillus subtilis. J Bacteriol. 1971 Mar;105(3):896–901. doi: 10.1128/jb.105.3.896-901.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoch J. A., Mathews J. L. Chromosomal location of pleiotropic negative sporulation mutations in Bacillus subtilis. Genetics. 1973 Feb;73(2):215–228. doi: 10.1093/genetics/73.2.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hoch J. A. Selection of cells transformed to prototrophy for sporulation markers. J Bacteriol. 1971 Mar;105(3):1200–1201. doi: 10.1128/jb.105.3.1200-1201.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ionesco H., Michel J., Cami B., Schaeffer P. Symposium on bacterial spores: II. Genetics of sporulation in Bacillus subtilis Marburg. J Appl Bacteriol. 1970 Mar;33(1):13–24. doi: 10.1111/j.1365-2672.1970.tb05230.x. [DOI] [PubMed] [Google Scholar]
  11. Kay D., Warren S. C. Sporulation in Bacillus subtilis. Morphological changes. Biochem J. 1968 Oct;109(5):819–824. doi: 10.1042/bj1090819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LACKS S., HOTCHKISS R. D. A study of the genetic material determining an enzyme in Pneumococcus. Biochim Biophys Acta. 1960 Apr 22;39:508–518. doi: 10.1016/0006-3002(60)90205-5. [DOI] [PubMed] [Google Scholar]
  13. MAHLER I., NEUMANN I. M., MARMUR J. Studies of genetic-units controlling arginine biosynthesis in Bacillus subtilis. Biochim Biophys Acta. 1963 May 28;72:69–79. [PubMed] [Google Scholar]
  14. Millet J., Ryter A. Mutants de Bacillus subtilis Marburg bloqués tardivement dans leur sporulation. Ann Inst Pasteur (Paris) 1972 Mar;122(3):395–406. [PubMed] [Google Scholar]
  15. Piggot P. J. Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporeulation operons. J Bacteriol. 1973 Jun;114(3):1241–1253. doi: 10.1128/jb.114.3.1241-1253.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rogolsky M. Chromosomal regions which control sporulation in Bacillus subtilis. Can J Microbiol. 1969 Jul;15(7):787–790. doi: 10.1139/m69-137. [DOI] [PubMed] [Google Scholar]
  17. SCHAEFFER P., IONESCO H. [Contribution to the genetic study of bacterial sporogenesis]. C R Hebd Seances Acad Sci. 1960 Dec 19;251:3125–3127. [PubMed] [Google Scholar]
  18. Sterlini J. M., Mandelstam J. Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem J. 1969 Jun;113(1):29–37. doi: 10.1042/bj1130029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. TAKAHASHI I. LOCALIZATION OF SPORE MARKERS ON THE CHROMOSOME OF BACILLUS SUBTILIS. J Bacteriol. 1965 Apr;89:1065–1067. doi: 10.1128/jb.89.4.1065-1067.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES