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. 1974 Sep;119(3):726–735. doi: 10.1128/jb.119.3.726-735.1974

Con− Mutants: Class of Mutants in Escherichia coli K-12 Lacking a Major Cell Wall Protein and Defective in Conjugation and Adsorption of a Bacteriophage

Ronald A Skurray 1, Robert E W Hancock 1, Peter Reeves 1
PMCID: PMC245674  PMID: 4604263

Abstract

We describe a new class of mutants of Escherichia coli K-12 defective in conjugation (Con− mutants). They lack a major protein of the outer membrane and are defective as recipients with Hfr and F′ donors and as recipients for several F-like R factors and Col factors. In the case of crosses with an Hfr donor, we have shown that the Con− recipient is defective in pair formation. The mutants are resistant to certain phages due to loss of receptor activity.

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Selected References

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