Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Sep;119(3):771–775. doi: 10.1128/jb.119.3.771-775.1974

Fatty Acids Present in the Lipopolysaccharide of Rhizobium trifolii

R Russa 1, Z Lorkiewicz 1
PMCID: PMC245680  PMID: 4852028

Abstract

Approximately 70% of the fatty acids recovered after acid or alkaline hydrolysis of the lipopolysaccharide of Rhizobium trifolii were hydroxy fatty acids identified as hydroxymyristic and hydroxypalmitic acids. Palmitic acid was the only saturated fatty acid found in the lipopolysaccharide of R. trifolii. Octadecenoic and a small amount of hexadecenoic acids were also identified. The results of BF3 methanolysis and hydroxylaminolysis suggest that hydroxypalmitic acid is N-acyl bound.

Full text

PDF
771

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams G. A., Tornabene T. G., Yaguchi M. Cell wall lipopolysaccharides from Neisseria catarrhalis. Can J Microbiol. 1969 Apr;15(4):365–374. doi: 10.1139/m69-067. [DOI] [PubMed] [Google Scholar]
  2. Bishop D. G., Hewett M. J., Knox K. W. Biochemical studies on lipopolysaccharides of Veillonella. Eur J Biochem. 1971 Mar 11;19(2):169–175. doi: 10.1111/j.1432-1033.1971.tb01301.x. [DOI] [PubMed] [Google Scholar]
  3. Björndal H., Erbing C., Lindberg B., Fåhraeus G., Ljunggren H. Studies on an extracellular polysaccharide from Rhizobium meliloti. Acta Chem Scand. 1971;25(4):1281–1286. doi: 10.3891/acta.chem.scand.25-1281. [DOI] [PubMed] [Google Scholar]
  4. Bunn C. R., McNeill J. J., Elkan G. H. Effect of biotin on fatty acids and phospholipids of biotin-sensitive strains of Rhizobium japonicum. J Bacteriol. 1970 Apr;102(1):24–29. doi: 10.1128/jb.102.1.24-29.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DUDMAN W. F. GROWTH AND EXTRACELLULAR POLYSACCHARIDE PRODUCTION BY RHIZOBIUM MELILOTI IN DEFINED MEDIUM. J Bacteriol. 1964 Sep;88:640–645. doi: 10.1128/jb.88.3.640-645.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graham P. H., O'Brien M. A. Composition of lipopolysaccharides from Rhizobium and Agrobacterium. Antonie Van Leeuwenhoek. 1968;34(3):326–330. doi: 10.1007/BF02046454. [DOI] [PubMed] [Google Scholar]
  7. HUMPHREY B. A., VINCENT J. M. Extracellular polysaccharides of Rhizobium. J Gen Microbiol. 1959 Dec;21:477–484. doi: 10.1099/00221287-21-3-477. [DOI] [PubMed] [Google Scholar]
  8. Hancock I. C., Humphreys G. O., Meadow P. M. Characterisation of the hydroxy acids of Pseudomonas aeruginosa 8602. Biochim Biophys Acta. 1970 Mar 10;202(2):389–391. doi: 10.1016/0005-2760(70)90204-3. [DOI] [PubMed] [Google Scholar]
  9. Humphrey B., Vincent J. M. The somatic antigens of two strains of Rhizobium trifolii. J Gen Microbiol. 1969 Dec;59(3):411–425. doi: 10.1099/00221287-59-3-411. [DOI] [PubMed] [Google Scholar]
  10. KANESHIRO T., MARR A. G. Hydroxy fatty acids of Azotobacter agilis. Biochim Biophys Acta. 1963 Jun 18;70:271–277. doi: 10.1016/0006-3002(63)90751-0. [DOI] [PubMed] [Google Scholar]
  11. Rietschel E. T., Gottert H., Lüderitz O., Westphal O. Nature and linkages of the fatty acids present in the lipid-A component of Salmonella lipopolysaccharides. Eur J Biochem. 1972 Jul 13;28(2):166–173. doi: 10.1111/j.1432-1033.1972.tb01899.x. [DOI] [PubMed] [Google Scholar]
  12. SNYDER F., STEPHENS N. A simplified spectrophotometric determination of ester groups in lipids. Biochim Biophys Acta. 1959 Jul;34:244–245. doi: 10.1016/0006-3002(59)90255-0. [DOI] [PubMed] [Google Scholar]
  13. Wilkinson S. G., Galbraith L., Lightfoot G. A. Cell walls, lipids, and lipopolysaccharides of Pseudomonas species. Eur J Biochem. 1973 Feb 15;33(1):158–174. doi: 10.1111/j.1432-1033.1973.tb02666.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES