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. 1974 Sep;119(3):848–856. doi: 10.1128/jb.119.3.848-856.1974

Characterization of Salmonella typhimurium Strains Sensitive and Resistant to Methionine Sulfoximine

Kathelyn Steimer-Veale a,1, Jean E Brenchley a
PMCID: PMC245690  PMID: 4152809

Abstract

Methionine sulfoximine inhibits the growth of Salmonella typhimurium at a concentration of 50 μM, and the addition of glutamine, but not glutamate, is sufficient to overcome this inhibition. The analogue causes 50% inhibition of glutamine synthetase activity at 2 to 4 μM and of glutamate synthase at 2 to 3 mM when these enzymes are assayed in vitro. No inhibition of glutamate dehydrogenase activity is observed at analogue concentrations as high as 50 mM. Two mutants selected for their resistance to methionine sulfoximine inhibition have a partial growth requirement for glutamine and a reduction in the glutamine synthetase and glutamate synthase activities. The sensitivity of the remaining glutamine synthetase activity in these mutants to methionine sulfoximine inhibition appears unaltered, and the lesions conferring the analogue resistance may not affect glutamine synthetase directly.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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