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. 1974 Sep;119(3):913–922. doi: 10.1128/jb.119.3.913-922.1974

Surface Polysaccharide from Staphylococcus aureus M That Contains Taurine, d-Aminogalacturonic Acid, and d-Fucosamine

Deng-Fong Liau 1, M Ann Melly 1, John H Hash 1
PMCID: PMC245699  PMID: 4854538

Abstract

The surface polysaccharide antigen of Staphylococcus aureus M was isolated, and the component parts were identified. The antigen is composed of taurine, d-aminogalacturonic acid, and d-fucosamine. The capsule is chemically distinct from all previously reported staphylococcal capsules. Taurine and aminogalacturonic acid have not been reported previously in staphylococci.

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Selected References

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  1. BARKER S. A., BRIMACOMBE J. S., HOW M. J., STACEY M., WILLIAMS J. M. Two new amino-sugars from an antigenic polysaccharide of Pneumococcus. Nature. 1961 Jan 28;189:303–304. doi: 10.1038/189303a0. [DOI] [PubMed] [Google Scholar]
  2. Baddiley J. The Leeuwenhoek lecture, 1967. Teichoic acids and the molecular structure of bacterial walls. Proc R Soc Lond B Biol Sci. 1968 Jul 9;170(1021):331–348. doi: 10.1098/rspb.1968.0043. [DOI] [PubMed] [Google Scholar]
  3. CRUMPTON M. J., DAVIES D. A. The isolation of D-fucosamine from the specific polysaccharide of Chromobacterium violaceum (NCTC 7917). Biochem J. 1958 Dec;70(4):729–736. doi: 10.1042/bj0700729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FISHER M. W., DEVLIN H. B., ERLANDSON A. L. A NEW STAPHYLOCOCCAL ANTIGEN. ITS PREPARATION AND IMMUNIZING ACTIVITY AGAINST EXPERIMENTAL INFECTIONS. Nature. 1963 Sep 14;199:1074–1075. doi: 10.1038/1991074a0. [DOI] [PubMed] [Google Scholar]
  5. Fletcher P. L., Jr, Hash J. H. Ribonuclease of Chalaropsis species. I. Isolation and physical properties. Biochemistry. 1972 Nov 7;11(23):4274–4280. doi: 10.1021/bi00773a013. [DOI] [PubMed] [Google Scholar]
  6. HANESSIAN S., HASKELL T. H. STRUCTURAL STUDIES ON STAPHYLOCOCCAL POLYSACCHARIDE ANTIGEN. J Biol Chem. 1964 Sep;239:2758–2764. [PubMed] [Google Scholar]
  7. HASKELL T. H., HANESSIAN S. THE PURIFICATION AND CHARACTERIZATION OF A NEW ACTIVE IMMUNIZING POLYSACCHARIDE PREPARED FROM STAPHYLOCOCCUS AUREUS. Biochim Biophys Acta. 1964 Mar 2;83:35–41. doi: 10.1016/0926-6526(64)90048-5. [DOI] [PubMed] [Google Scholar]
  8. Hash J. H., Rothlauf M. V. The N,O-diacetylmuramidase of Chalaropsis species. I. Purification and crystallization. J Biol Chem. 1967 Dec 10;242(23):5586–5590. [PubMed] [Google Scholar]
  9. Hurst R. E. The trimethylsilylation reactions of hexosamines, and gas-chromatographic separation of the derivatives. Carbohydr Res. 1973 Sep;30(1):143–154. doi: 10.1016/s0008-6215(00)82181-3. [DOI] [PubMed] [Google Scholar]
  10. Jann B., Jann K., Beyaert G. O. 2-Amino-2,6-dideoxy-d-glucose (D-quinovosamine): a constituent of the lipopolysaccharides of Vibrio cholerae. Eur J Biochem. 1973 Sep 3;37(3):531–534. doi: 10.1111/j.1432-1033.1973.tb03015.x. [DOI] [PubMed] [Google Scholar]
  11. KELLY A. P., WEED L. L. TAURINE AS A CONSTITUENT OF A BACTERIAL CELL WALL. J Biol Chem. 1965 Jun;240:2519–2523. [PubMed] [Google Scholar]
  12. Melly M. A., Duke L. J., Liau D. F., Hash J. H. Biological properties of the encapsulated Staphylococcus aureus M. Infect Immun. 1974 Aug;10(2):389–397. doi: 10.1128/iai.10.2.389-397.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PERKINS H. R. A polymer containing glucose and aminohexuronic acid isolated from the cell walls of micrococcus lysodeikticus. Biochem J. 1963 Mar;86:475–483. doi: 10.1042/bj0860475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SCHINDLER C. A., SCHUHARDT V. T. LYSOSTAPHIN: A NEW BACTERIOLYTIC AGENT FOR THE STAPHYLOCOCCUS. Proc Natl Acad Sci U S A. 1964 Mar;51:414–421. doi: 10.1073/pnas.51.3.414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. STOFFYN P. J., JEANLOZ R. W. Identification of amino sugars by paper chromatography. Arch Biochem Biophys. 1954 Oct;52(2):373–379. doi: 10.1016/0003-9861(54)90137-x. [DOI] [PubMed] [Google Scholar]
  16. Sandford P. A., Watson P. R., Jeanes A. R. An extracellular microbial polysaccharide composed of 2-acetamido-2-deoxy-D-glucose and 2-acetamido-2-deoxy-D-glucuronic acid: radiochemical and gas-chromatographic analysis of the products of methanolysis. Carbohydr Res. 1973 Jul;29(1):153–164. doi: 10.1016/s0008-6215(00)82079-0. [DOI] [PubMed] [Google Scholar]
  17. Scott A. C. A capsulate Staphylococcus aureus. J Med Microbiol. 1969 Aug;2(3):253–260. doi: 10.1099/00222615-2-3-253. [DOI] [PubMed] [Google Scholar]
  18. Seiler N. Use of the dansyl reaction in biochemical analysis. Methods Biochem Anal. 1970;18:259–337. doi: 10.1002/9780470110362.ch5. [DOI] [PubMed] [Google Scholar]
  19. Sharon N., Shif I., Zehavi U. The isolation of D-fucosamine (2-amino-2,6-dideoxy-D-galactose) from polysaccharides of Bacillus. Biochem J. 1964 Oct;93(1):210–214. doi: 10.1042/bj0930210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shih J. W., Hash J. H. The N,O-diacetylmuramidase of Chalaropsis species. 3. Amino acid composition and partial structural formula. J Biol Chem. 1971 Feb 25;246(4):994–1006. [PubMed] [Google Scholar]
  21. Smith E. J. Purification and properties of an acidic polysaccharide isolated from Achromobacter georgiopolitanum. J Biol Chem. 1968 Oct 10;243(19):5139–5144. [PubMed] [Google Scholar]
  22. TREVELYAN W. E., PROCTER D. P., HARRISON J. S. Detection of sugars on paper chromatograms. Nature. 1950 Sep 9;166(4219):444–445. doi: 10.1038/166444b0. [DOI] [PubMed] [Google Scholar]
  23. Vanaman T. C., Wakil S. J., Hill R. L. The preparation of tryptic, peptic, thermolysin, and cyanogen bromide peptides from the acyl carrier protein of Escherichia coli. J Biol Chem. 1968 Dec 25;243(24):6409–6419. [PubMed] [Google Scholar]
  24. WHEAT R., ROLLINS E. L., LEATHERWOOD J. ISOLATION OF D-FUCOSAMINE FROM BACILLUS CEREUS. Nature. 1964 May 2;202:492–493. doi: 10.1038/202492a0. [DOI] [PubMed] [Google Scholar]
  25. WILLIAMSON A. R., ZAMENHOF S. The type-specific substance of Hemophilus influenzae, type d: the natural occurrence of glucosamine uronic acid. J Biol Chem. 1963 Jul;238:2255–2258. [PubMed] [Google Scholar]
  26. Wong K. H., Feeley J. C. Isolation of Vi antigen and a simple method for its measurement. Appl Microbiol. 1972 Oct;24(4):628–633. doi: 10.1128/am.24.4.628-633.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wu T. C., Park J. T. Chemical characterization of a new surface antigenic polysaccharide from a mutant of Staphylococcus aureus. J Bacteriol. 1971 Nov;108(2):874–884. doi: 10.1128/jb.108.2.874-884.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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