Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Oct;120(1):43–51. doi: 10.1128/jb.120.1.43-51.1974

Genetic Mapping of the Antigenic Determinants of Two Polysaccharide K Antigens, K10 and K54, in Escherichia coli

Ida Ørskov a, Kate Nyman a,1
PMCID: PMC245728  PMID: 4138850

Abstract

The genes controlling synthesis of the Escherichia coli acidic polysaccharide capsular antigens K10 and K54 were transferred by conjugation to E. coli strains of other serotypes. The genes concerned with these K antigen determinants showed genetic linkage with the serA locus. We propose to name the K antigen-controlling gene kpsA. The genetic determinants of the two K antigens could also be transferred to enteropathogenic serotypes, even though such strains have never been found in nature with special acidic polysaccharide K antigens. A noncapsulated derivative, K−, of the K10 strain can transfer the genetic determinant of the K antigen, demonstrating the probable existence of another chromosomal locus involved in the production of such acidic polysaccharide K antigens.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brenner D. J., Falkow S. Genetics of the Enterobacteriaceae. C. Molecular relationships among members of the Enterobacteriaceae. Adv Genet. 1971;16:81–118. doi: 10.1016/s0065-2660(08)60355-7. [DOI] [PubMed] [Google Scholar]
  2. Demerec M, Fano U. Bacteriophage-Resistant Mutants in Escherichia Coli. Genetics. 1945 Mar;30(2):119–136. doi: 10.1093/genetics/30.2.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Duguid J. P., Anderson E. S., Campbell I. Fimbriae and adhesive properties in Salmonellae. J Pathol Bacteriol. 1966 Jul;92(1):107–138. doi: 10.1002/path.1700920113. [DOI] [PubMed] [Google Scholar]
  4. Formal S. B., Gemski P., Baron L. S., Labrec E. H. Genetic Transfer of Shigella flexneri Antigens to Escherichia coli K-12. Infect Immun. 1970 Mar;1(3):279–287. doi: 10.1128/iai.1.3.279-287.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glynn A. A., Howard C. J. The sensitivity to complement of strains of Escherichia coli related to their K antigens. Immunology. 1970 Mar;18(3):331–346. [PMC free article] [PubMed] [Google Scholar]
  6. HENRIKSEN S. D. Cross-reacting M-antigens in Escherichia coli and Salmonella paratyphi B. Acta Pathol Microbiol Scand. 1950;27(1):107–109. doi: 10.1111/j.1699-0463.1950.tb05199.x. [DOI] [PubMed] [Google Scholar]
  7. JACOB F., WOLLMAN E. L. Analyse des groupes de liaison génétique de différentes souches donatrices d'Escherichia coli K 12. C R Hebd Seances Acad Sci. 1957 Nov 18;245(21):1840–1843. [PubMed] [Google Scholar]
  8. JOHNSON E. M., FALKOW S., BARON L. S. CHROMOSOME TRANSFER KINETICS OF SALMONELLA HFR STRAINS. J Bacteriol. 1964 Aug;88:395–400. doi: 10.1128/jb.88.2.395-400.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jann K., Jann B., Orskov F., Orskov I., Westphal O. Immunchemische Untersuchungen an K-Antigenen von Escherichia Coli. II. Das K-Antigen von E. coli 08:K42(A):H-. Biochem Z. 1965 Jun 3;342(1):1–22. [PubMed] [Google Scholar]
  10. Johnson E. M., Baron L. S. Genetic transfer of the Vi antigen from Salmonella typhosa to Escherichia coli. J Bacteriol. 1969 Jul;99(1):358–359. doi: 10.1128/jb.99.1.358-359.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lieberman M. M., Markovitz A. Depression of guanosine diphosphate-mannose pyrophosphorylase by mutations in two different regulator genes involved in capsular polysaccharide synthesis in Escherichia coli K-12. J Bacteriol. 1970 Mar;101(3):965–972. doi: 10.1128/jb.101.3.965-972.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lieberman M. M., Shaparis A., Markovitz A. Control of uridine diphosphate-glucose dehydrogenase synthesis and uridine diphosphate-glucuronic acid accumulation by a regulator gene mutation in Escherichia coli K-12. J Bacteriol. 1970 Mar;101(3):959–964. doi: 10.1128/jb.101.3.959-964.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MARKOVITZ A. REGULATORY MECHANISMS FOR SYNTHESIS OF CAPSULAR POLYSACCHARIDE IN MUCOID MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1964 Feb;51:239–246. doi: 10.1073/pnas.51.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Markovitz A., Lieberman M. M., Rosenbaum N. Derepression of phosphomannose isomerase by regulator gene mutations involved in capsular polysaccharide synthesis in Escherichia coli K-12. J Bacteriol. 1967 Nov;94(5):1497–1501. doi: 10.1128/jb.94.5.1497-1501.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Markovitz A., Sydiskis R. J., Lieberman M. M. Genetic and biochemical studies on mannose-negative mutants that are deficient in phosphomannose isomerase in Escherichia coli K-12. J Bacteriol. 1967 Nov;94(5):1492–1496. doi: 10.1128/jb.94.5.1492-1496.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Miyake T. Exchange of Genetic Material between Salmonella Typhimurium and Escherichia Coli K-12. Genetics. 1962 Aug;47(8):1043–1052. doi: 10.1093/genetics/47.8.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mäkelä P. H. Inheritance of the O antigens of Salmonella groups B and D. J Gen Microbiol. 1965 Oct;41(1):57–65. doi: 10.1099/00221287-41-1-57. [DOI] [PubMed] [Google Scholar]
  18. ORSKOV F., ORSKOV I. The fertility of Escherichia coli antigen test strains in crosses with K 12. Acta Pathol Microbiol Scand. 1961;51:280–290. [PubMed] [Google Scholar]
  19. ORSKOV I., ORSKOV F., JANN B., JANN K. ACIDIC POLYSACCHARIDE ANTIGENS OF A NEW TYPE FROM E. COLI CAPSULES. Nature. 1963 Oct 12;200:144–146. doi: 10.1038/200144a0. [DOI] [PubMed] [Google Scholar]
  20. Orskov F., Orskov I. Immunoelectrophoretic patterns of extracts from Escherichia coli O antigen test strains O1 to O157 examinations in homologous OK sera. Further comments on the classification of Escherichia K antigens. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(6):905–910. [PubMed] [Google Scholar]
  21. Orskov F., Orskov I., Jann B., Jann K. Immunoelectrophoretic patterns of extracts from all Escherichia coli O and K antigen test strains: correlation with pathogenicity. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(2):142–152. doi: 10.1111/j.1699-0463.1971.tb02141.x. [DOI] [PubMed] [Google Scholar]
  22. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  23. Sanderson K. E. Genetics of the Enterobacteriaceae. A. Genetic homology in the Enterobacteriaceae. Adv Genet. 1971;16:35–51. doi: 10.1016/s0065-2660(08)60353-3. [DOI] [PubMed] [Google Scholar]
  24. Stirm S., Orskov F., Orskov I., Birch-Andersen A. Episome-carried surface antigen K88 of Escherichia coli. 3. Morphology. J Bacteriol. 1967 Feb;93(2):740–748. doi: 10.1128/jb.93.2.740-748.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stocker B. A., Wilkinson R. G., Mäkelä P. H. Genetic aspects of biosynthesis and structure of Salmonella somatic polysaccharide. Ann N Y Acad Sci. 1966 Jun 30;133(2):334–348. doi: 10.1111/j.1749-6632.1966.tb52375.x. [DOI] [PubMed] [Google Scholar]
  26. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. UMBARGER H. E., UMBARGER M. A., SIU P. M. BIOSYNTHESIS OF SERINE IN ESCHERICHIA COLI AND SALMONELLA TYPHIMURIUM. J Bacteriol. 1963 Jun;85:1431–1439. doi: 10.1128/jb.85.6.1431-1439.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Uerkvitz W., Karlström O., Munch-Petersen A. A deoxyuridine monophosphate phosphatase detected in mutants of Escherichia coli lacking alkaline phosphatase and 5'-nucleotidase. Mol Gen Genet. 1973 Mar 19;121(4):337–346. doi: 10.1007/BF00433232. [DOI] [PubMed] [Google Scholar]
  29. ØRSKOV F. Studies on a number of Escherichia coli strains belonging to O group 86. Acta Pathol Microbiol Scand. 1954;35(2):179–186. doi: 10.1111/j.1699-0463.1954.tb00860.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES