Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Oct;120(1):245–252. doi: 10.1128/jb.120.1.245-252.1974

Promoter-Like Mutation Affecting HPr and Enzyme I of the Phosphoenolpyruvate:Sugar Phosphotransferase System in Salmonella typhimurium1

J Christopher Cordaro a, R Philip Anderson a,2, E Wayne Grogan Jr a,3, Donald J Wenzel a,4, Michael Engler a, Saul Roseman a
PMCID: PMC245757  PMID: 4608878

Abstract

A promoter-like mutation, ptsP160, has been identified which drastically reduces expression of the genes specifying two proteins, HPr and enzyme I, of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in Salmonella typhimurium. This mutation lies between trzA, a gene specifying susceptibility to 1,2,4-triazole, and ptsH, the structural gene for HPr. It leads to a loss of active transport of those sugars that require the PTS for entry into the cell. Pseudorevertants of strains carrying this promoter-like mutation have additional lesions very closely linked to ptsP160 by transduction analysis and are noninducible for HPr and enzyme I above a basal level. Presumably, strains carrying ptsP160 are defective in the normal induction mechanism for HPr and enzyme I, and the pseudorevertants derived from them result from second-site initiation signals within or near this promoter-like element. The induction of HPr and enzyme I above their noninduced levels apparently is not required for transport of at least one PTS sugar, methyl α-d-glucopyranoside, since this sugar is taken up by the pseudorevertants at the same rate as by the wild type. The existence of a promoter-like element governing the coordinate inducibility of both HPr and enzyme I suggests that ptsH and ptsI constitute an operon. Wild-type levels of a sugar-specific PTS protein, factor III, are synthesized in response to the crr+ gene in both a ptsP160 strain and its pseudorevertants; this suggests that the crr+ gene has its own promoter distinct from ptsP.

Full text

PDF
245

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson B., Weigel N., Kundig W., Roseman S. Sugar transport. 3. Purification and properties of a phosphocarrier protein (HPr) of the phosphoenolpyruvate-dependent phosphotransferase system of Escherichia coli. J Biol Chem. 1971 Nov 25;246(22):7023–7033. [PubMed] [Google Scholar]
  2. Boro H., Brenchley J. E. A new generalized transducing phage for Salmonella typhimurium LT2. Virology. 1971 Sep;45(3):835–836. doi: 10.1016/0042-6822(71)90208-x. [DOI] [PubMed] [Google Scholar]
  3. Cordaro J. C., Balbinder E. Evidence for the separability of the operator from the first structural gene in the tryptophan operon of Salmonella typhimurium. Genetics. 1971 Feb;67(2):151–169. doi: 10.1093/genetics/67.2.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cordaro J. C., Roseman S. Deletion mapping of the genes coding for HPr and enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system in Salmonella typhimurium. J Bacteriol. 1972 Oct;112(1):17–29. doi: 10.1128/jb.112.1.17-29.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KUNDIG W., GHOSH S., ROSEMAN S. PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM. Proc Natl Acad Sci U S A. 1964 Oct;52:1067–1074. doi: 10.1073/pnas.52.4.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kundig W., Roseman S. Sugar transport. I. Isolation of a phosphotransferase system from Escherichia coli. J Biol Chem. 1971 Mar 10;246(5):1393–1406. [PubMed] [Google Scholar]
  8. Kundig W., Roseman S. Sugar transport. II. Characterization of constitutive membrane-bound enzymes II of the Escherichia coli phosphotransferase system. J Biol Chem. 1971 Mar 10;246(5):1407–1418. [PubMed] [Google Scholar]
  9. Ota N., Galsworthy P. R., Pardee A. B. Genetics of sulfate transport by Salmonella typhimurium. J Bacteriol. 1971 Mar;105(3):1053–1062. doi: 10.1128/jb.105.3.1053-1062.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saier M. H., Jr, Simoni R. D., Roseman S. The physiological behavior of enzyme I and heat-stable protein mutants of a bacterial phosphotransferase system. J Biol Chem. 1970 Nov 10;245(21):5870–5873. [PubMed] [Google Scholar]
  11. Saier M. H., Roseman S. Inducer exclusion and repression of enzyme synthesis in mutants of Salmonella typhimurium defective in enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system. J Biol Chem. 1972 Feb 10;247(3):972–975. [PubMed] [Google Scholar]
  12. Schwartz D. O., Beckwith J. R. Mutagens which cause deletions in Escherichia coli. Genetics. 1969 Feb;61(2):371–376. doi: 10.1093/genetics/61.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Simoni R. D., Levinthal M., Kundig F. D., Kundig W., Anderson B., Hartman P. E., Roseman S. Genetic evidence for the role of a bacterial phosphotransferase system in sugar transport. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1963–1970. doi: 10.1073/pnas.58.5.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith H. O., Levine M. A phage P22 gene controlling integration of prophage. Virology. 1967 Feb;31(2):207–216. doi: 10.1016/0042-6822(67)90164-x. [DOI] [PubMed] [Google Scholar]
  15. Stock J., Roseman S. A sodium-dependent sugar co-transport system in bacteria. Biochem Biophys Res Commun. 1971 Jul 2;44(1):132–138. doi: 10.1016/s0006-291x(71)80168-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES