Abstract
Loss of photoreversibility (LOP) of the ultraviolet (UV) damage which prevents reinitiation of deoxyribonucleic acid (DNA) replication occurred with incubation of Escherichia coli B/r thy trp cultures after UV doses of 240, 320, and 400 ergs/mm2. LOP occurred at the time of reinitiation of DNA replication in the cultures (i.e., after postirradiation lag periods of 45 min or more). Neither the absence of thymine nor the absence of tryptophan prevented LOP of the damage to DNA replication, suggesting that neither DNA replication nor protein synthesis is necessary for the process. These findings suggest that attempted initiation of DNA replication results in transformation of pyrimidine damage into permanent damage to chromosome structure at the reinitiation site.
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