Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Oct;120(1):527–531. doi: 10.1128/jb.120.1.527-531.1974

Dark-Field Electron Microscopy of Thin Sections of Trichosporon cutaneum

Claes Weibull 1
PMCID: PMC245793  PMID: 4213592

Abstract

Dark-field electron micrographs of aldehyde-fixed, unstained yeast-phase cells of Trichosporon cutaneum exhibit remarkable contrast and good resolution when the sections studied are less than 25 nm thick and conical illumination is used.

Full text

PDF
527

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brakenhoff G. J., Nanninga N., Pieters J. Relative mass determination from darkfield electron micrographs with an application to ribosomes. J Ultrastruct Res. 1972 Nov;41(3):238–257. doi: 10.1016/s0022-5320(72)90067-6. [DOI] [PubMed] [Google Scholar]
  2. Dubochet J., Ducommun M., Zollinger M., Kellenberger E. A new preparation method for dark-field electron microscopy of biomacromolecules. J Ultrastruct Res. 1971 Apr;35(1):147–167. doi: 10.1016/s0022-5320(71)80148-x. [DOI] [PubMed] [Google Scholar]
  3. Fukami A., Adachi K. A new method of preparation of a self-perforated micro plastic grid and its application. J Electron Microsc (Tokyo) 1965;14(2):112–118. [PubMed] [Google Scholar]
  4. Neujahr H. Y., Varga J. M. Degradation of phenols by intact cells and cell-free preparations of Trichosporon cutaneum. Eur J Biochem. 1970 Mar 1;13(1):37–44. doi: 10.1111/j.1432-1033.1970.tb00896.x. [DOI] [PubMed] [Google Scholar]
  5. Weibull C. Electron microscope studies on aldehyde-fixed, unstained microbial cells. J Ultrastruct Res. 1973 Apr;43(1):150–159. doi: 10.1016/s0022-5320(73)90075-0. [DOI] [PubMed] [Google Scholar]
  6. Weibull C. Estimation of the thickness of films used in electron microscopy. Z Allg Mikrobiol. 1972;12(6):487–490. doi: 10.1002/jobm.3630120607. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES