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. 1974 Nov;120(2):631–637. doi: 10.1128/jb.120.2.631-637.1974

Subcellular Localization of Isoleucine-Valine Biosynthetic Enzymes in Yeast1

E D Ryan a, G B Kohlhaw a
PMCID: PMC245821  PMID: 4616942

Abstract

By using the method of stepwise homogenization of yeast spheroplast lysates employed previously with the leucine biosynthetic enzymes, it is shown that threonine deaminase, acetohydroxy acid synthase, Mg2+-dependent isomero-reductase, and dihydroxy acid dehydratase are particulate. Density gradient centrifugation and the behavior of marker enzymes suggest that all of the above enzymes of the isoleucine-valine biosynthetic pathway are associated with the mitochondria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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