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. 1974 Nov;120(2):679–686. doi: 10.1128/jb.120.2.679-686.1974

Transduction and Plasmid Deoxyribonucleic Acid Analysis in a Multiply Antibiotic-Resistant Strain of Staphylococcus epidermidis

Linda L Rosendorf 1, Fritz H Kayser 1
PMCID: PMC245827  PMID: 4281773

Abstract

A genetic analysis of a multiply antibiotic-resistant strain of Staphylococcus epidermidis was performed. Experiments designed to show reversion of organisms to antibiotic susceptibility, as well as studies of the influence of ultraviolet irradiation of phage on the transduction frequencies of the resistance markers, indicated that determinants of chloramphenicol (cml), tetracycline (tet), and neomycin (neo) resistance are present on separate plasmids, but the streptomycin marker is chromosomal. In 2 to 6% of tetracycline-resistant transductants, co-transduction of cml was also observed. By using CsCl-dye density gradients followed by neutral sucrose gradients, the plasmids carrying cml, tet, and neo could be isolated and their molecular weights could be determined. The tetracycline plasmid is shown to be incompatible with one of the cryptic plasmids of a recipient strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andriole V. T., Lyons R. W. Coagulase-negative staphylococcus. Ann N Y Acad Sci. 1970 Oct 30;174(2):533–544. doi: 10.1111/j.1749-6632.1970.tb45579.x. [DOI] [PubMed] [Google Scholar]
  2. BAIRD-PARKER A. C. A classification of micrococci and staphylococci based on physiological and biochemical tests. J Gen Microbiol. 1963 Mar;30:409–427. doi: 10.1099/00221287-30-3-409. [DOI] [PubMed] [Google Scholar]
  3. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  4. Bentley D. W., Hahn J. J., Lepper M. H. Transmission of chloramphenicol-resistant Staphylococcus epidermidis: epidemiologic and laboratory studies. J Infect Dis. 1970 Nov;122(5):365–375. doi: 10.1093/infdis/122.5.365. [DOI] [PubMed] [Google Scholar]
  5. Clowes R. C. Molecular structure of bacterial plasmids. Bacteriol Rev. 1972 Sep;36(3):361–405. doi: 10.1128/br.36.3.361-405.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen S., Sweeney H. M. Transduction of Methicillin Resistance in Staphylococcus aureus Dependent on an Unusual Specificity of the Recipient Strain. J Bacteriol. 1970 Dec;104(3):1158–1167. doi: 10.1128/jb.104.3.1158-1167.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Freifelder D. Electron microscopic study of the ethidium bromide-DNA complex. J Mol Biol. 1971 Sep 14;60(2):401–403. doi: 10.1016/0022-2836(71)90303-2. [DOI] [PubMed] [Google Scholar]
  8. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Holt R. Coagulase-negative staphylococci as opportunist pathogens. Arzneimittelforschung. 1971 Mar;21(3):325–328. [PubMed] [Google Scholar]
  10. Ikeda H., Tomizawa J. Prophage P1, and extrachromosomal replication unit. Cold Spring Harb Symp Quant Biol. 1968;33:791–798. doi: 10.1101/sqb.1968.033.01.091. [DOI] [PubMed] [Google Scholar]
  11. KORMAN R. Z., BERMAN D. T. Medium for the differentiation of acid producing colonies of staphylococci. J Bacteriol. 1958 Oct;76(4):454–455. doi: 10.1128/jb.76.4.454-455.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kayser F. H., Wiemer U. Uber die Oxacillinresistenz pathogener Staphylokokken. I. Die Natur der Resistenz gegen Oxyacillin bei Staphylococcus pyogenes aureus. Z Hyg Infektionskr. 1965;150(4):308–316. [PubMed] [Google Scholar]
  13. Kayser F. H., Wüst J., Corrodi P. Transduction and elimination of resistance determinants in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 1972 Sep;2(3):217–223. doi: 10.1128/aac.2.3.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Keys T. F., Hewitt W. L. Endocarditis due to Micrococci and Staphylococcus epidermidis. Arch Intern Med. 1973 Aug;132(2):216–220. [PubMed] [Google Scholar]
  15. MACHATTIE L. A., BERNE K. I., THOMAS C. A., Jr ELECTRON MICROSCOPY OF DNA FROM HEMOPHILUS INFLUENZAE. J Mol Biol. 1965 Mar;11:648–649. doi: 10.1016/s0022-2836(65)80019-5. [DOI] [PubMed] [Google Scholar]
  16. Marsik F. J., Parisi J. T. Significance of Staphylococcus epidermidis in the clinical laboratory. Appl Microbiol. 1973 Jan;25(1):11–14. doi: 10.1128/am.25.1.11-14.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Minshew B. H., Rosenblum E. D. Plasmid for tetracycline resistance in Staphylococcus epidermidis. Antimicrob Agents Chemother. 1973 May;3(5):568–574. doi: 10.1128/aac.3.5.568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NOVICK R. P., RICHMOND M. H. NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965 Aug;90:467–480. doi: 10.1128/jb.90.2.467-480.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Novick R. P., Bouanchaud D. The problems of drug-resistant pathogenic bacteria. Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann N Y Acad Sci. 1971 Jun 11;182:279–294. doi: 10.1111/j.1749-6632.1971.tb30664.x. [DOI] [PubMed] [Google Scholar]
  20. Novick R. P., Roth C. Plasmid-linked resistance to inorganic salts in Staphylococcus aureus. J Bacteriol. 1968 Apr;95(4):1335–1342. doi: 10.1128/jb.95.4.1335-1342.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. PERRET C. J. Iodometric assay of penicillinase. Nature. 1954 Nov 27;174(4439):1012–1013. doi: 10.1038/1741012a0. [DOI] [PubMed] [Google Scholar]
  22. Pulverer G., Damen G., Neugebauer M. Antibiotic resistance of Staphylococcus albus. Med Microbiol Immunol. 1972;158(1):32–43. doi: 10.1007/BF02122006. [DOI] [PubMed] [Google Scholar]
  23. Schaefler S. Polyfunctional penicillinase plasmid in Staphylococcus epidermidis: bacteriophage restriction and modification mutants. J Bacteriol. 1972 Nov;112(2):697–706. doi: 10.1128/jb.112.2.697-706.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Verhoef J., Van Boven C. P., Winkler K. C. Phage-typing of coagulase-negative staphylococci. J Med Microbiol. 1972 Feb;5(1):9–19. doi: 10.1099/00222615-5-1-9. [DOI] [PubMed] [Google Scholar]

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