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. 1974 Nov;120(2):822–830. doi: 10.1128/jb.120.2.822-830.1974

In Vivo Kinetics of Nitrogenase Formation in Clostridium pasteurianum

Belinda Seto a,1, L E Mortenson a
PMCID: PMC245844  PMID: 4218235

Abstract

Clostridium pasteurianum exhibits diauxic growth when grown in the presence of both NH3 and N2; no nitrogenase activity or formation was detected either serologically or by activity during growth on NH3. During the 60-min lag that ensued after NH3 was consumed and before growth resumed, molybdoferredoxin and azoferredoxin were first detected by activity measurements and serologically at 25 and 40 min, respectively. With the use of rifampin and dactinomycin, it was found that azoferredoxin messenger ribonucleic acid was initiated between 25 and 30 min after the inception of the lag and was completed by 38 min. An explanation of these results and their relation to possible models for the regulation of nitrogenase is given.

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Selected References

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