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. 1974 Nov;120(2):831–836. doi: 10.1128/jb.120.2.831-836.1974

5-Bromouridylyl-(3′ → 5′)-Adenosine Isolated from 5-Bromouracil-Induced Filaments of Escherichia coli

Roosevelt J Jones a, Dorothy P Thompson a,1
PMCID: PMC245845  PMID: 4376142

Abstract

A dinucleoside monophosphate was isolated from 5-bromouracil-induced filaments of a thymine auxotroph of Escherichia coli K-12. The dinucleoside monophosphate was fractioned from a [14C]5-bromouracil-labeled perchloric acid extract using Dowex-1-formate ion-exchange chromatography. Sephadex chromatography revealed its molecular weight to be 710. Snake venom phosphodiesterase digest of the dinucleoside monophosphate yielded [14C]5-bromouridine and adenosine 5′-monophosphate. The presence of [14C]5-bromouracil in bacterial ribonucleic acid indicates that ribonucleic acid, which had incorporated 5-bromouracil, was the probable source of this dinucleoside monophosphate, 5-bromouridylyl-(3′ → 5′)-adenosine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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