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. 1974 Nov;120(2):861–865. doi: 10.1128/jb.120.2.861-865.1974

Stimulation of Transport into Escherichia coli Membrane Vesicles by Internally Generated Reduced Nicotinamide Adenine Dinucleotide

Masamitsu Futai a,1
PMCID: PMC245849  PMID: 4156360

Abstract

Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about 60 to 80% of that stimulated by d-lactate. This transport was inhibited by cyanide and anaerobiosis. Ferricyanide, a nonpermeable electron acceptor, inhibited transport stimulated by external NADH, but not that stimulated by internally generated NADH.

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Selected References

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