Abstract
Induction of a heat-inducible abnormal lambda prophage inserted between lysA and thyA in Escherichia coli resulted in a number of auxotrophic mutants in the surviving cured-cell populations. These mutants could not be accounted for by deletions arising on formation of lambda hybrid particles carrying regions adjacent to the insertion site. The properties of these mutants, which were almost all spontaneously revertable, have been described and mapped by F′ episome complementation. Tentatively, it was suggested that induction of the lambda lysogen leads to a mutagenic state.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Böck A., Neidhardt F. C. Location of the structural gene for glycyl ribonucleic acid synthetase by means of a strain of Escherichia coli possessing an unusual enzyme. Z Vererbungsl. 1966;98(3):187–192. doi: 10.1007/BF00888946. [DOI] [PubMed] [Google Scholar]
- Chung S. T., Greenberg G. R. Loss of an essential function of Escherichia coli by deletions in the thyA region. J Bacteriol. 1973 Dec;116(3):1145–1149. doi: 10.1128/jb.116.3.1145-1149.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Folk W. R., Berg P. Isolation and partial characterization of Escherichia coli mutants with altered glycyl transfer ribonucleic acid synthetases. J Bacteriol. 1970 Apr;102(1):193–203. doi: 10.1128/jb.102.1.193-203.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Itikawa H., Baumberg S., Vogel H. J. Enzymic basis for a genetic suppression: accumulation and deacylation of N-acetylglutamic gamma-semialdehyde in enterobacterial mutants. Biochim Biophys Acta. 1968 Jul 9;159(3):547–550. doi: 10.1016/0005-2744(68)90142-3. [DOI] [PubMed] [Google Scholar]
- Kuo T. T., Stocker B. A. Suppression of proline requirement of proA and proAB deletion mutants in Salmonella typhimurium by mutation to arginine requirement. J Bacteriol. 1969 May;98(2):593–598. doi: 10.1128/jb.98.2.593-598.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K12. J Mol Biol. 1973 Oct 25;80(2):297–314. doi: 10.1016/0022-2836(73)90174-5. [DOI] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]