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. 1974 Dec;120(3):1268–1273. doi: 10.1128/jb.120.3.1268-1273.1974

Lipids of the Spirochaetales: Comparison of the Lipids of Several Members of the Genera Spirochaeta, Treponema, and Leptospira

Brian P Livermore a,1, Russell C Johnson a
PMCID: PMC245910  PMID: 4436256

Abstract

The lipid compositions of 17 spirochetes belonging to the genera Spirochaeta and Treponema were investigated and compared with data previously derived from 11 strains of Leptospira. The lipid compositions and lipid metabolism of any of these genera is sufficiently different to be characteristic of that genus and to differentiate it from the other two genera. Members of the genus Leptospira are characterized by their ability to beta-oxidize long chain fatty acids as their major carbon and energy source. With few exceptions, they are incapable of synthesizing fatty acids de novo. The major phospholipid found was phosphatidyl ethanolamine. No glycolipid or phosphatidyl choline was found in these organisms. Members of the genus Treponema studied were incapable of beta-oxidation as well as de novo synthesis of fatty acids. Phosphatidyl choline is the major phospholipid of this genus. The glycolipid, monogalactosyl diglyceride, is a major component of the Treponema. Members of the Spirochaeta did synthesize fatty acids de novo. Although these spirochetes contain a monoglycosyl diglyceride, the hexose content of the glycolipid varied from species to species. Neither phosphatidyl ethanolamine nor phosphatidyl choline was found in the Spirochaeta.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Breznak J. A., Canale-Parola E. Spirochaeta aurantia, a pigmented, facultatively anaerobic spirochete. J Bacteriol. 1969 Jan;97(1):386–395. doi: 10.1128/jb.97.1.386-395.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hespell R. B., Canale-Parola E. Carbohydrate metabolism in Spirochaeta stenostrepta. J Bacteriol. 1970 Jul;103(1):216–226. doi: 10.1128/jb.103.1.216-226.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. JACIN H., MISHKIN A. R. SEPARATION OF CARBOHYDRATES ON BORATE-IMPREGNATED SILICA GEL G PLATES. J Chromatogr. 1965 Apr;18:170–173. doi: 10.1016/s0021-9673(01)80341-1. [DOI] [PubMed] [Google Scholar]
  5. Johnson R. C., Eggebraten L. M. Fatty Acid Requirements of the Kazan 5 and Reiter Strains of Treponema pallidum. Infect Immun. 1971 Jun;3(6):723–726. doi: 10.1128/iai.3.6.723-726.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johnson R. C., Livermore B. P., Jenkin H. M., Eggebraten L. Lipids of Treponema pallidum Kazan 5. Infect Immun. 1970 Nov;2(5):606–609. doi: 10.1128/iai.2.5.606-609.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johnson R. C., Livermore B. P., Walby J. K., Jenkin H. M. Lipids of parasitic and saprophytic leptospires. Infect Immun. 1970 Sep;2(3):286–291. doi: 10.1128/iai.2.3.286-291.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johnson R. C., Walby J. K. Cultivation of leptospires: fatty acid requirements. Appl Microbiol. 1972 May;23(5):1027–1028. doi: 10.1128/am.23.5.1027-1028.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Joseph R. Fatty acid composition of Spirochaeta stenostrepta. J Bacteriol. 1972 Oct;112(1):629–631. doi: 10.1128/jb.112.1.629-631.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Livermore B. P., Johnson R. C. Isolation and characterization of a glycolipid from Treponema pallidum, Kazan 5. Biochim Biophys Acta. 1970 Jul 14;210(2):315–318. doi: 10.1016/0005-2760(70)90176-1. [DOI] [PubMed] [Google Scholar]
  11. Livermore B. P., Johnson R. C., Jenkin H. M. Isolation of an unusual positional isomer of hexadecenoic acid from a parasitic leptospire. Lipids. 1969 Mar;4(2):166–167. doi: 10.1007/BF02531939. [DOI] [PubMed] [Google Scholar]
  12. Meyer H., Meyer F. Lipid metabolism in the parasitic and free-living spirochetes Treponema pallidum (Reiter) and Treponema zuelzerae. Biochim Biophys Acta. 1971 Feb 2;231(1):93–106. doi: 10.1016/0005-2760(71)90257-8. [DOI] [PubMed] [Google Scholar]

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