Abstract
Cells of the mixotrophic chemolithotroph (facultative autotroph) Thiobacillus intermedius which have been grown on a glucose-yeast extract medium, a condition in which glucose is used as a source of energy, accumulate the non-metabolizable analogue 2-deoxy-d-glucose against a concentration gradient in a predominantly unchanged state. On the other hand, cells grown mixotrophically on a thiosulfate-glucose medium, a condition in which glucose provides cell carbon but is not used extensively for energy, and in which enzymes of the Entner-Doudoroff pathway are repressed, do not accumulate 2-deoxy-d-glucose significantly. Similarly, cells grown chemolithotrophically on thiosulfate-carbonate do not take up this sugar. Transfer of thiosulfate-yeast extract-grown cells, which lack the capacity to accumulate 2-deoxy-d-glucose, to a glucose-yeast extract medium results in the induction of the concentrative sugar uptake system. The capacity of induced cells to take up 2-deoxy-d-glucose is inhibited by thiosulfate. Thus, the transport system for glucose appears to be regulated in this organism so that the sugar is accumulated only under conditions where it is utilized as a source of energy, and the presence of the preferred energy source leads to both repression and inhibition of the uptake system.
Full text
PDF





Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aleem M. I. Thiosulfate Oxidation and Electron Transport in Thiobacillus novellus. J Bacteriol. 1965 Jul;90(1):95–101. doi: 10.1128/jb.90.1.95-101.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blackkolb F., Schlegel H. G. Regulation der Glucose-6-phosphate-Dehydrogenase aus Hydrogenomonas H 16 durch ATP und NADH. Arch Mikrobiol. 1968;63(2):177–196. [PubMed] [Google Scholar]
- GOTTSCHALK G. DIE VERWERTUNG ORGANISCHER SUBSTRATE DURCH HYDROGENOMONAS IN GEGENWART VON MOLEKULAREM WASSERSTOFF. Biochem Z. 1965 Feb 24;341:260–270. [PubMed] [Google Scholar]
- Guymon L. F., Eagon R. G. Transport of glucose, gluconate, and methyl alpha-D-glucoside by Pseudomonas aeruginosa. J Bacteriol. 1974 Mar;117(3):1261–1269. doi: 10.1128/jb.117.3.1261-1269.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaback H. R. Transport across isolated bacterial cytoplasmic membranes. Biochim Biophys Acta. 1972 Aug 4;265(3):367–416. doi: 10.1016/0304-4157(72)90014-7. [DOI] [PubMed] [Google Scholar]
- Krulwich T. A., Ensign J. C. Alteration of glucose metabolism of Arthrobacter crystallopoietes by compounds which induce sphere to rod morphogenesis. J Bacteriol. 1969 Feb;97(2):526–534. doi: 10.1128/jb.97.2.526-534.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- London J., Rittenberg S. C. Effects of organic matter on the growth of Thiobacillus intermedius. J Bacteriol. 1966 Mar;91(3):1062–1069. doi: 10.1128/jb.91.3.1062-1069.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matin A., Rittenberg S. C. Regulation of glucose metabolism in Thiobacillus intermedius. J Bacteriol. 1970 Oct;104(1):239–246. doi: 10.1128/jb.104.1.239-246.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matin A., Rittenberg S. C. Utilization of glucose in heterotrophic media by Thiobacillus intermedius. J Bacteriol. 1970 Oct;104(1):234–238. doi: 10.1128/jb.104.1.234-238.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Midgley M., Dawes E. A. The regulation of transport of glucose and methyl alpha-glucoside in Pseudomonas aeruginosa. Biochem J. 1973 Feb;132(2):141–154. doi: 10.1042/bj1320141. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mukkada A. J., Long G. L., Romano A. H. The uptake of 2-deoxy-D-glucose by Pseudomonas aeruginosa and its regulation. Biochem J. 1973 Feb;132(2):155–162. doi: 10.1042/bj1320155. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ng F. M., Dawes E. A. Chemostat studies on the regulation of glucose metabolism in Pseudomonas aeruginosa by citrate. Biochem J. 1973 Feb;132(2):129–140. doi: 10.1042/bj1320129. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Romano A. H., Eberhard S. J., Dingle S. L., McDowell T. D. Distribution of the phosphoenolpyruvate: glucose phosphotransferase system in bacteria. J Bacteriol. 1970 Nov;104(2):808–813. doi: 10.1128/jb.104.2.808-813.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TRUDINGER P. A. Thiosulphate oxidation and cytochromes in Thiobacillus X. 2. Thiosulphate-oxidizing enzyme. Biochem J. 1961 Apr;78:680–686. doi: 10.1042/bj0780680. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tabita R., Lundgren D. G. Utilization of glucose and the effect of organic compounds on the chemolithotroph Thiobacillus ferrooxidans. J Bacteriol. 1971 Oct;108(1):328–333. doi: 10.1128/jb.108.1.328-333.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tiwari N. P., Campbell J. J. Enzymatic control of the metabolic activity of Pseudomonas aeruginosa grown in glucose or succinate media. Biochim Biophys Acta. 1969 Dec 30;192(3):395–401. doi: 10.1016/0304-4165(69)90388-2. [DOI] [PubMed] [Google Scholar]
- Winkler H. H. A hexose-phosphate transport system in Escherichia coli. Biochim Biophys Acta. 1966 Mar 28;117(1):231–240. doi: 10.1016/0304-4165(66)90170-x. [DOI] [PubMed] [Google Scholar]