Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1975 Feb;121(2):740–742. doi: 10.1128/jb.121.2.740-742.1975

Nucleotide pools and regulation of ribonucleic acid synthesis in yeast.

R Kudrna, G Edlin
PMCID: PMC245992  PMID: 1089641

Abstract

Nucleotide pools were measured in growing and amino acid-starved Saccharomyces cerevisiae. During amino acid starvation there are neither significant changes in the endogeneous nucleoside triphosphate pool levels nor measurable synthesis of guanosine 5'-diphosphate, 3'-diphosphate. Stable ribonucleic acid synthesis does not appear to be regulated by changes in the triphosphate pools or by the unusual nucleotide guanosine 5'-diphosphate, 3'-diphosphate.

Full text

PDF
740

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARONSON A. I., SPIEGELMAN S. Protein and ribonucleic acid synthesis in a chloramphenicol-inhibited system. Biochim Biophys Acta. 1961 Oct 14;53:70–84. doi: 10.1016/0006-3002(61)90795-8. [DOI] [PubMed] [Google Scholar]
  2. Bailey R. B., Parks L. W. Response of the intracellular adenosine triphosphate pool of Saccharomyces cerevisiae to growth inhibition induced by excess L-methionine. J Bacteriol. 1972 Aug;111(2):542–546. doi: 10.1128/jb.111.2.542-546.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beck C., Ingraham J., Maaloe O., Neuhard J. Relationship between the concentration of nucleoside triphosphates and the rate of synthesis of RNA. J Mol Biol. 1973 Jun 25;78(1):117–121. doi: 10.1016/0022-2836(73)90431-2. [DOI] [PubMed] [Google Scholar]
  4. Cashel M., Gallant J. Two compounds implicated in the function of the RC gene of Escherichia coli. Nature. 1969 Mar 1;221(5183):838–841. doi: 10.1038/221838a0. [DOI] [PubMed] [Google Scholar]
  5. Cashel M., Kalbacher B. The control of ribonucleic acid synthesis in Escherichia coli. V. Characterization of a nucleotide associated with the stringent response. J Biol Chem. 1970 May 10;245(9):2309–2318. [PubMed] [Google Scholar]
  6. Cashel M., Lazzarini R. A., Kalbacher B. An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate. J Chromatogr. 1969 Mar 11;40(1):103–109. doi: 10.1016/s0021-9673(01)96624-5. [DOI] [PubMed] [Google Scholar]
  7. Colby C., Edlin G. Nucleotide pool levels in growing, inhibited, and transformed chick fibroblast cells. Biochemistry. 1970 Feb 17;9(4):917–920. doi: 10.1021/bi00806a029. [DOI] [PubMed] [Google Scholar]
  8. Edlin G., Broda P. Physiology and genetics of the "ribonucleic acid control" locus in escherichia coli. Bacteriol Rev. 1968 Sep;32(3):206–226. doi: 10.1128/br.32.3.206-226.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Edlin G., Neuhard J. Regulation of nucleoside triphosphate pools in Escherichia coli. J Mol Biol. 1967 Mar 14;24(2):225–230. doi: 10.1016/0022-2836(67)90328-2. [DOI] [PubMed] [Google Scholar]
  10. Gallant J., Harada B. The control of ribonucleic acid synthesis in Escherichia coli. 3. The functional relationship between purine ribonucleoside triphosphate pool sizes and the rate of ribonucleic acid accumulation. J Biol Chem. 1969 Jun 25;244(12):3125–3132. [PubMed] [Google Scholar]
  11. Indge K. J. Polyphosphates of the yeast cell vacuole. J Gen Microbiol. 1968 May;51(3):447–455. doi: 10.1099/00221287-51-3-447. [DOI] [PubMed] [Google Scholar]
  12. KURLAND C. G., MAALOE O. Regulation of ribosomal and transfer RNA synthesis. J Mol Biol. 1962 Mar;4:193–210. doi: 10.1016/s0022-2836(62)80051-5. [DOI] [PubMed] [Google Scholar]
  13. McLaughlin C. S., Magee P. T., Hartwell L. H. Role of isoleucyl-transfer ribonucleic acid synthetase in ribonucleic acid synthesis and enzyme repression in yeast. J Bacteriol. 1969 Nov;100(2):579–584. doi: 10.1128/jb.100.2.579-584.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Que L., Jr, Willie G. R., Cashel M., Bodley J. W., Gray G. R. Guanosine 5'-diphosphate, 3'-diphosphate: assignment of structure by 13C nuclear magnetic resonance spectroscopy. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2563–2566. doi: 10.1073/pnas.70.9.2563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Roth R. M., Dampier C. Dependence of ribonucleic acid synthesis on continuous protein synthesis in yeast. J Bacteriol. 1972 Feb;109(2):773–779. doi: 10.1128/jb.109.2.773-779.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. SIEGEL M. R., SISLER H. D. SITE OF ACTION OF CYCLOHEXIMIDE IN CELLS OF SACCHAROMYCES PASTORIANUS. II. THE NATURE OF INHIBITION OF PROTEIN SYNTHESIS IN A CELL-FREE SYSTEM. Biochim Biophys Acta. 1964 May 18;87:83–89. [PubMed] [Google Scholar]
  17. STENT G. S., BRENNER S. A genetic locus for the regulation of ribonucleic acid synthesis. Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005–2014. doi: 10.1073/pnas.47.12.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Siegel M. R., Sisler H. D. Site of action of cycloheximide in cells of Saccharomyces pastorianus. 3. Further studies on the mechanism of action and the mechanism of resistance in saccharomyces species. Biochim Biophys Acta. 1965 Aug 10;103(4):558–567. [PubMed] [Google Scholar]
  19. Sy J., Lipmann F. Identification of the synthesis of guanosine tetraphosphate (MS I) as insertion of a pyrophosphoryl group into the 3'-position in guanosine 5'-diphosphate. Proc Natl Acad Sci U S A. 1973 Feb;70(2):306–309. doi: 10.1073/pnas.70.2.306. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wehr C. T., Parks L. W. Macromolecular synthesis in Saccharomyces cerevisiae in different growth media. J Bacteriol. 1969 May;98(2):458–466. doi: 10.1128/jb.98.2.458-466.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weisblum B., Davies J. Antibiotic inhibitors of the bacterial ribosome. Bacteriol Rev. 1968 Dec;32(4 Pt 2):493–528. [PMC free article] [PubMed] [Google Scholar]
  22. Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES