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. 1975 Mar;121(3):771–776. doi: 10.1128/jb.121.3.771-776.1975

Interactions between exogenous deoxyribonucleic acid and membrane vesicles isolated from competent and noncompetent Bacillus subtilis.

H Joenje, W N Konings, G Venema
PMCID: PMC246002  PMID: 803962

Abstract

Competent cultures of Bacillus subtilis 168 have been fractionated into a high-competent and a low-competent fraction by a large-scale separation technique. Membrane vesicles isolated from both cell fractions are equally active in the transport of L-glutamate. Both membrane vesicle preparations seem to have similar endo- and exonuclease activities. Also, both preparations are capable of binding deoxyribonucleic acid. However, especially at low deoxyribonucleic acid concentrations (1 mug or less per ml), vesicles obtained from competent cells bind significantly more deoxyribonucleic acid (up to sixfold) than vesicles from noncompetent cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akrigg A., Ayad S. R., Blamire J. Uptake of DNA by competent bacteria--a possible mechanism. J Theor Biol. 1969 Sep;24(3):266–272. doi: 10.1016/s0022-5193(69)80051-2. [DOI] [PubMed] [Google Scholar]
  2. Arwert F., Venema G. Transformation in Bacillus subtilis. Fate of newly introduced transforming DNA. Mol Gen Genet. 1973;123(2):185–198. doi: 10.1007/BF00267334. [DOI] [PubMed] [Google Scholar]
  3. Cahn F. H., Fox M. S. Fractionation of transformable bacteria from ocompetent cultures of Bacillus subtilis on renografin gradients. J Bacteriol. 1968 Mar;95(3):867–875. doi: 10.1128/jb.95.3.867-875.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davidoff-Abelson R., Dubnau D. Kinetic analysis of the products of donor deoxyribonucleate in transformed cells of Bacillus subtilis. J Bacteriol. 1973 Oct;116(1):154–162. doi: 10.1128/jb.116.1.154-162.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dubnau D., Cirigliano C. Fate of transforming DNA following uptake by competent Bacillus subtilis. Formation and properties of products isolated from transformed cells which are derived entirely from donor DNA. J Mol Biol. 1972 Feb 28;64(1):9–29. doi: 10.1016/0022-2836(72)90318-x. [DOI] [PubMed] [Google Scholar]
  6. Hadden C., Nester E. W. Purification of competent cells in the Bacillus subtilis transformation system. J Bacteriol. 1968 Mar;95(3):876–885. doi: 10.1128/jb.95.3.876-885.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Joenje H., Konings W. N., Venema G. Interactions between exogenous deoxyribonucleic acid and membrane vesicles isolated from Bacillus subtilis 168. J Bacteriol. 1974 Sep;119(3):784–794. doi: 10.1128/jb.119.3.784-794.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Konings W. N., Bisschop A., Veenhuis M., Vermeulen C. A. New procedure for the isolation of membrane vesicles of Bacillus subtilis and an electron microscopy study of their ultrastructure. J Bacteriol. 1973 Dec;116(3):1456–1465. doi: 10.1128/jb.116.3.1456-1465.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Konings W. N., Freese E. Amino acid transport in membrane vesicles of Bacillus subtilis. J Biol Chem. 1972 Apr 25;247(8):2408–2418. [PubMed] [Google Scholar]
  10. Seto H., Tomasz A. Early stages in DNA binding and uptake during genetic transformation of pneumococci. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1493–1498. doi: 10.1073/pnas.71.4.1493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Seto H., Tomasz A. Protoplast formation and leakage of intramembrane cell components: induction by the competence activator substance of pneumococci. J Bacteriol. 1975 Jan;121(1):344–353. doi: 10.1128/jb.121.1.344-353.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vermeulen C. A., Venema G. Autoradiographic estimation of competence and the relationship between competence and transformability in cultures of Bacillus subtilis. J Gen Microbiol. 1971 Dec;69(2):239–252. doi: 10.1099/00221287-69-2-239. [DOI] [PubMed] [Google Scholar]
  14. Vermeulen C. A., Venema G. Electron microscope and autoradiographic study of ultrastructural aspects of competence and deoxyribonucleic acid absorption in Bacillus subtilis: ultrastructure of competent and noncompetent cells and cellular changes during development of competence. J Bacteriol. 1974 May;118(2):334–341. doi: 10.1128/jb.118.2.334-341.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wilson G. A., Bott K. F. Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1968 Apr;95(4):1439–1449. doi: 10.1128/jb.95.4.1439-1449.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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