Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1975 Mar;121(3):950–958. doi: 10.1128/jb.121.3.950-958.1975

New transfer ribonucleic acid species during sporulation of Bacillus subtilis.

Y H Jeng, R H Doi
PMCID: PMC246023  PMID: 803966

Abstract

The transfer ribonucleic acid (tRNA) populations from log-phase cells, sporulating cells (stage III), and dormant spores were compared by tRNA-deoxyribonucleic acid hybridization techniques. New tRNA species not found in log-phase cells were observed in stage III cells. Some of the tRNA made during sporulation were also present in dormant spores. Although the role and function of these new tRNA species cannot be ascribed directly to the sporulation process, their presence indicates that new tRNA genes can be transcribed during sporulation and suggests that translational control may be exerted during sporulation by tRNA.

Full text

PDF
950

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arceneaux J. L., Sueoka N. Two species of Bacillus subtilis tyrosine transfer ribonucleic acid. Biological properties and alteration in their relative amounts during growth. J Biol Chem. 1969 Nov 10;244(21):5959–5966. [PubMed] [Google Scholar]
  2. Bishop J. O., Robertson F. W., Burns J. A., Melli M. Methods for the analysis of deoxyribonucleic acid-ribonucleic acid hybridization data. Biochem J. 1969 Nov;115(3):361–370. doi: 10.1042/bj1150361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DiCioccio R. A., Strauss N. Patterns of transcription in Bacillus subtilis during sporulation. J Mol Biol. 1973 Jun 25;77(2):325–336. doi: 10.1016/0022-2836(73)90338-0. [DOI] [PubMed] [Google Scholar]
  4. Doi R. H., Kaneko I., Igarashi R. T. Pattern of valine transfer ribonucleic acid of Bacillus subtilis under different growth conditions. J Biol Chem. 1968 Mar 10;243(5):945–951. [PubMed] [Google Scholar]
  5. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  6. Hough B. R., Davidson E. H. Studies on the repetitive sequence transcripts of Xenopus oocytes. J Mol Biol. 1972 Oct 14;70(3):491–509. doi: 10.1016/0022-2836(72)90555-4. [DOI] [PubMed] [Google Scholar]
  7. KIRBY K. S. A new method for the isolation of ribonucleic acids from mammalian tissues. Biochem J. 1956 Nov;64(3):405–408. doi: 10.1042/bj0640405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaneko I., Doi R. H. Alteration of valyl-sRNA during sporulation of bacillus subtilis. Proc Natl Acad Sci U S A. 1966 Mar;55(3):564–571. doi: 10.1073/pnas.55.3.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lazzarini R. A. Differences in lysine-sRNA from spore and vegetative cells of Bacillus subtillis. Proc Natl Acad Sci U S A. 1966 Jul;56(1):185–190. doi: 10.1073/pnas.56.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Margulies L., Remeza V., Rudner R. Asymmetric template function of microbial deoxyribonucleic acids: transcription of ribosomal and soluble ribonucleic acids. J Bacteriol. 1970 Sep;103(3):560–568. doi: 10.1128/jb.103.3.560-568.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McConaughy B. L., Laird C. D., McCarthy B. J. Nucleic acid reassociation in formamide. Biochemistry. 1969 Aug;8(8):3289–3295. doi: 10.1021/bi00836a024. [DOI] [PubMed] [Google Scholar]
  12. Oishi M. The transcribing strands of bacillus subtilis DNA for ribosomal and transfer RNA. Proc Natl Acad Sci U S A. 1969 Jan;62(1):256–262. doi: 10.1073/pnas.62.1.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RALPH R. K., BELLAMY A. R. ISOLATION AND PURIFICATION OF UNDEGRADED RIBONUCLEIC ACIDS. Biochim Biophys Acta. 1964 May 18;87:9–16. doi: 10.1016/0926-6550(64)90041-6. [DOI] [PubMed] [Google Scholar]
  14. Smith K. D., Armstrong J. L., McCarthy B. J. The introduction of radioisotopes into RNA by methylation in vitro. Biochim Biophys Acta. 1967 Jul 18;142(2):323–330. doi: 10.1016/0005-2787(67)90615-6. [DOI] [PubMed] [Google Scholar]
  15. Sueoka N., Kano-Sueoka T. Transfer RNA and cell differentiation. Prog Nucleic Acid Res Mol Biol. 1970;10:23–55. doi: 10.1016/s0079-6603(08)60560-7. [DOI] [PubMed] [Google Scholar]
  16. Sumida-Yasumoto C., Doi R. H. Transcription from the complementary deoxyribonucleic acid strands of Bacillus subtilis during various stages of sporulation. J Bacteriol. 1974 Feb;117(2):775–782. doi: 10.1128/jb.117.2.775-782.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vold B. S. Comparison of lysyl-transfer ribonucleic acid species from vegetative cells and spores of Bacillus subtilis by methylated albumin-kieselguhr and reversed-phase chromatography. J Bacteriol. 1970 Jun;102(3):711–715. doi: 10.1128/jb.102.3.711-715.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yamakawa T., Doi R. H. Preferential transcription of Bacillus subtilis light deoxyribonucleic acid strands during sporulation. J Bacteriol. 1971 May;106(2):305–310. doi: 10.1128/jb.106.2.305-310.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES