Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1975 Mar;121(3):1000–1006. doi: 10.1128/jb.121.3.1000-1006.1975

F deoxyribonucleic acid transferred to recipient cells in the presence of rifampin.

S Hiraga, T Saitoh
PMCID: PMC246029  PMID: 1090582

Abstract

When Escherichia coli F+ cells resistant to rifampin and streptomycin are mated with F- cells sensitive to the antibiotics, a large fraction of F factor deoxyribonucleic acid (DNA), which is transferred to the recipient cells in the presence of either of the antibiotics, can be recovered as covalently closed, circular double-stranded DNA, as in the control mating in the absence of antibiotic. Similar results were obtained in the presence of chloramphenicol or chloramphenicol plus rifampin. It is suggested that the transferred single-stranded F DNA can be converted to the covalently closed, circular double-stranded form in the absence of protein synthesis, and that rifampin-sensitive transcription is not required for the conversion.

Full text

PDF
1000

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BONHOEFFER F., SCHALLER H. A METHOD FOR SELECTIVE ENRICHMENT OF MUTANTS BASED ON THE HIGH UV SENSITIVITY OF DNA CONTAINING 5-BROMOURACIL. Biochem Biophys Res Commun. 1965 Jun 18;20:93–97. [PubMed] [Google Scholar]
  2. Brutlag D., Schekman R., Kornberg A. A possible role for RNA polymerase in the initiation of M13 DNA synthesis. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826–2829. doi: 10.1073/pnas.68.11.2826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clewell D. B., Evenchik B. G. Effects of rifampicin, streptolydigin and actinomycin D on the replication of Col E1 plasmid DNA in Escherichia coli. J Mol Biol. 1973 Apr 15;75(3):503–513. doi: 10.1016/0022-2836(73)90457-9. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B., Evenchik B., Cranston J. W. Direct inhibition of Col E 1 plasmid DNA replication in Escherichia coli by rifampicin. Nat New Biol. 1972 May 3;237(70):29–31. doi: 10.1038/newbio237029a0. [DOI] [PubMed] [Google Scholar]
  5. Cohen A., Fisher W. D., Curtiss R., 3rd, Adler H. I. The properties of DNA transferred to minicells during conjugation. Cold Spring Harb Symp Quant Biol. 1968;33:635–641. doi: 10.1101/sqb.1968.033.01.071. [DOI] [PubMed] [Google Scholar]
  6. Curtiss R., 3rd, Caro L. G., Allison D. P., Stallions D. R. Early stages of conjugation in Escherichia coli. J Bacteriol. 1969 Nov;100(2):1091–1104. doi: 10.1128/jb.100.2.1091-1104.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fenwick R. G., Jr, Curtiss R., 3rd Conjugal deoxyribonucleic acid replication by Excherichia coli K-12: effect of chloramphenicol and rifampin. J Bacteriol. 1973 Dec;116(3):1224–1235. doi: 10.1128/jb.116.3.1224-1235.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. HERSHEY A. D., CHASE M. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J Gen Physiol. 1952 May;36(1):39–56. doi: 10.1085/jgp.36.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hinkle D. C., Richardson C. C. Bacteriophage T7 deoxyribonucleic acid replication in vitro. Requirements for deoxyribonucleic acid synthesis and characterization of the product. J Biol Chem. 1974 May 10;249(9):2974–2980. [PubMed] [Google Scholar]
  10. Hiraga S., Igarashi K., Yura T. A deoxythymidine kinase-deficient mutant of Escherichia coli. I. Isolation and some properties. Biochim Biophys Acta. 1967 Aug 22;145(1):41–51. doi: 10.1016/0005-2787(67)90652-1. [DOI] [PubMed] [Google Scholar]
  11. Hiraga S., Saitoh T. Initation of DNA replication in Escherichia coli. I. Characteristics of the initation process in dna mutants. Mol Gen Genet. 1974;132(1):49–62. doi: 10.1007/BF00268230. [DOI] [PubMed] [Google Scholar]
  12. Igarashi K., Hiraga S., Yura T. A deoxythymidine kinase deficient mutant of Escherichia coli. II. Mapping and transduction studies with phage phi 80. Genetics. 1967 Nov;57(3):643–654. doi: 10.1093/genetics/57.3.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Klein A., Powling A. Initiation of lambda DNA replication in vitro. Nat New Biol. 1972 Sep 20;239(90):71–73. doi: 10.1038/newbio239071a0. [DOI] [PubMed] [Google Scholar]
  14. Kline B. C. Inhibition of plasmid DNA replication by rifampin in Salmonella pullorum. Biochem Biophys Res Commun. 1972 Mar 24;46(6):2019–2025. doi: 10.1016/0006-291x(72)90753-x. [DOI] [PubMed] [Google Scholar]
  15. Kline B. C. Mechanism and biosynthetic requirements for F plasmid replication in Escherichia coli. Biochemistry. 1974 Jan 1;13(1):139–146. doi: 10.1021/bi00698a022. [DOI] [PubMed] [Google Scholar]
  16. Kline B. C. Role of DNA transcription in the initiation of Escherichia coli sex factor (F) DNA replication. Biochem Biophys Res Commun. 1973 Jan 23;50(2):280–288. doi: 10.1016/0006-291x(73)90837-1. [DOI] [PubMed] [Google Scholar]
  17. Lark K. G. Evidence for the direct involvement of RNA in the initiation of DNA replication in Escherichia coli 15T. J Mol Biol. 1972 Feb 28;64(1):47–60. doi: 10.1016/0022-2836(72)90320-8. [DOI] [PubMed] [Google Scholar]
  18. Laurent S. J. Initiation of deoxyribonucleic acid replication in a temperature-sensitive mutant of B. subtilis: evidence for a transcriptional step. J Bacteriol. 1973 Oct;116(1):141–145. doi: 10.1128/jb.116.1.141-145.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Messer W. Initiation of deoxyribonucleic acid replication in Escherichia coli B-r: chronology of events and transcriptional control of initiation. J Bacteriol. 1972 Oct;112(1):7–12. doi: 10.1128/jb.112.1.7-12.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Messing J., Staudenbauer W. L., Hofschneider P. H. Inhibition of minicircular DNA replication in Escherichia coli 15 by rifampicin. Nat New Biol. 1972 Aug 16;238(85):202–203. doi: 10.1038/newbio238202a0. [DOI] [PubMed] [Google Scholar]
  21. Novotny C. P., Taylor P. F., Lavin K. Effects of growth inhibitors and ultraviolet irradiation on F pili. J Bacteriol. 1972 Dec;112(3):1083–1089. doi: 10.1128/jb.112.3.1083-1089.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ohki M., Tomizawa J. Asymmetric transfer of DNA strands in bacterial conjugation. Cold Spring Harb Symp Quant Biol. 1968;33:651–658. doi: 10.1101/sqb.1968.033.01.074. [DOI] [PubMed] [Google Scholar]
  23. Rupp W. D., Ihler G. Strand selection during bacterial mating. Cold Spring Harb Symp Quant Biol. 1968;33:647–650. doi: 10.1101/sqb.1968.033.01.073. [DOI] [PubMed] [Google Scholar]
  24. Sakabe K., Okazaki R. A unique property of the replicating region of chromosomal DNA. Biochim Biophys Acta. 1966 Dec 21;129(3):651–654. doi: 10.1016/0005-2787(66)90088-8. [DOI] [PubMed] [Google Scholar]
  25. Sakakibara Y., Tomizawa J. I. Replication of colicin E1 plasmid DNA in cell extracts. Proc Natl Acad Sci U S A. 1974 Mar;71(3):802–806. doi: 10.1073/pnas.71.3.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sakakibara Y., Tomizawa J. Replication of colicin E1 plasmid DNA in cell extracts. II. Selective synthesis of early replicative intermediates. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1403–1407. doi: 10.1073/pnas.71.4.1403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Shizuya H., Richardson C. C. Synthesis of bacteriophage lambda DNA in vitro: requirement for O and P gene products. Proc Natl Acad Sci U S A. 1974 May;71(5):1758–1762. doi: 10.1073/pnas.71.5.1758. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Staudenbauer W. L., Hofschneider P. H. Replication of bacteriophage M 13: inhibition of single-strand DNA synthesis by rifampicin. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1634–1637. doi: 10.1073/pnas.69.6.1634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sugino A., Hirose S., Okazaki R. RNA-linked nascent DNA fragments in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1863–1867. doi: 10.1073/pnas.69.7.1863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Sugino A., Okazaki R. RNA-linked DNA fragments in vitro. Proc Natl Acad Sci U S A. 1973 Jan;70(1):88–92. doi: 10.1073/pnas.70.1.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  32. Vapnek D., Rupp W. D. Asymmetric segregation of the complementary sex-factor DNA strands during conjugation in Escherichia coli. J Mol Biol. 1970 Nov 14;53(3):287–303. doi: 10.1016/0022-2836(70)90066-5. [DOI] [PubMed] [Google Scholar]
  33. Wickner R. B., Wright M., Wickner S., Hurwitz J. Conversion of phiX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3233–3237. doi: 10.1073/pnas.69.11.3233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Wickner W., Brutlag D., Schekman R., Kornberg A. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form. Proc Natl Acad Sci U S A. 1972 Apr;69(4):965–969. doi: 10.1073/pnas.69.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES