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. 1975 Mar;121(3):1117–1121. doi: 10.1128/jb.121.3.1117-1121.1975

Synthesis of nitrate reductase components in chlorate-resistant mutants of Escherichia coli.

C H MacGregor
PMCID: PMC246043  PMID: 1090592

Abstract

Specific antibody to purified nitrate reductase from Escherichia coli was used to identify enzyme components present in mutants which lack functional nitrate reductase. chlA and B mutants contained all three subunits present in the wild-type enzyme. Different peptides with a broad range of molecular weights could be precipitated from chlCmutants, and chlE mutants contained either slightly degraded enzyme subunits or no precipitable protein. No mutants produced significant amounts of cytoplasmic enzyme. The chlA and B loci are suggested to function in the synthesis and attachment of a molybdenum-containing factor. The chlC locus is suggested to be the structural gene for nitrate reductase subunit A and chlE is suggested to be involved in the synthesis of the cytochrome b1 apoprotein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azoulay E., Puig J., Pichinoty F. Alteration of respiratory particles by mutation in Escherichia coli K 12. Biochem Biophys Res Commun. 1967 Apr 20;27(2):270–274. doi: 10.1016/s0006-291x(67)80073-1. [DOI] [PubMed] [Google Scholar]
  2. Goldberg A. L., Howell E. M., Li J. B., Martel S. B., Prouty W. F. Physiological significance of protein degradation in animal and bacterial cells. Fed Proc. 1974 Apr;33(4):1112–1120. [PubMed] [Google Scholar]
  3. Goldschmidt R. In vivo degradation of nonsense fragments in E. coli. Nature. 1970 Dec 19;228(5277):1151–1154. doi: 10.1038/2281151a0. [DOI] [PubMed] [Google Scholar]
  4. Guest J. R. Biochemical and genetic studies with nitrate reductase C-gene mutants of Escherichia coli. Mol Gen Genet. 1969;105(4):285–297. doi: 10.1007/BF00277583. [DOI] [PubMed] [Google Scholar]
  5. Haddock B. A., Schairer H. U. Electron-transport chains of Escherichia coli. Reconstitution of respiration in a 5-aminolaevulinic acid-requiring mutant. Eur J Biochem. 1973 May;35(1):34–45. doi: 10.1111/j.1432-1033.1973.tb02806.x. [DOI] [PubMed] [Google Scholar]
  6. MacGregor C. H. Anaerobic cytochrome b1 in Escherichia coli: association with and regulation of nitrate reductase. J Bacteriol. 1975 Mar;121(3):1111–1116. doi: 10.1128/jb.121.3.1111-1116.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MacGregor C. H., Schnaitman C. A. Alterations in the cytoplasmic membrane proteins of various chlorate-resistant mutants of Escherichia coli. J Bacteriol. 1971 Oct;108(1):564–570. doi: 10.1128/jb.108.1.564-570.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MacGregor C. H., Schnaitman C. A., Normansell D. E. Purification and properties of nitrate reductase from Escherichia coli K12. J Biol Chem. 1974 Aug 25;249(16):5321–5327. [PubMed] [Google Scholar]
  9. MacGregor C. H., Schnaitman C. A. Reconstitution of nitrate reductase activity and formation of membrane particles from cytoplasmic extracts of chlorate-resistant mutants of Escherichia coli. J Bacteriol. 1973 Jun;114(3):1164–1176. doi: 10.1128/jb.114.3.1164-1176.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. MacGregor C. H., Schnaitman C. A. Restoration of reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase activity of a Neurospora mutant by extracts of various chlorate-resistant mutants of Escherichia coli. J Bacteriol. 1972 Oct;112(1):388–391. doi: 10.1128/jb.112.1.388-391.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MacGregor C. H. Solubilization of Escherichia coli nitrate reductase by a membrane-bound protease. J Bacteriol. 1975 Mar;121(3):1102–1110. doi: 10.1128/jb.121.3.1102-1110.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nason A., Lee K. Y., Pan S. S., Ketchum P. A., Lamberti A., DeVries J. Invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a Neurospora mutant and a component of molybdenum-enzymes. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3242–3246. doi: 10.1073/pnas.68.12.3242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stiefel E. I. Proposed molecular mechanism for the action of molybedenum in enzymes: coupled proton and electron transfer. Proc Natl Acad Sci U S A. 1973 Apr;70(4):988–992. doi: 10.1073/pnas.70.4.988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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