Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1975 Mar;121(3):1145–1157. doi: 10.1128/jb.121.3.1145-1157.1975

Incorporation of long-chain fatty acids of the substrate organism by Bdellovibrio bacteriovorus during intraperiplasmic growth.

J G Kuenen, S C Rittenberg
PMCID: PMC246047  PMID: 1090595

Abstract

Data are presented showing that a large proportion of the fatty acids of Bdellovibrio bacteriovorus grown intraperiplasmically are derived unaltered from the fatty acids of its substrate organism. Those fatty acids of the bdellovibrio not homologous with those of the substrate organism are derived mainly by metabolic alteration of preexisting fatty acids in the latter. De novo synthesis from acetate occurs only to a small extent. These characteristics of bdellovibrio physiology are in part responsible for its minimal energy expenditure for intraperiplasmic growth. The data presented also indicate that B. bacteriovorus is capable of hydrogenating unsaturated fatty acids, of beta-oxidation of fatty acids, and of regulating the proportion of saturated and unsaturated fatty acids in the lipids.

Full text

PDF
1145

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Diedrich D. L., Denny C. F., Hashimoto T., Conti S. F. Facultatively parasitic strain of Bdellovibrio bacteriovorus. J Bacteriol. 1970 Mar;101(3):989–996. doi: 10.1128/jb.101.3.989-996.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Forrest W. W., Walker D. J. The generation and utilization of energy during growth. Adv Microb Physiol. 1971;5:213–274. doi: 10.1016/s0065-2911(08)60408-7. [DOI] [PubMed] [Google Scholar]
  4. Hespell R. B., Rosson R. A., Thomashow M. F., Rittenberg S. C. Respiration of Bdellovibrio bacteriovorus strain 109J and its energy substrates for intraperiplasmic growth. J Bacteriol. 1973 Mar;113(3):1280–1288. doi: 10.1128/jb.113.3.1280-1288.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hespell R. B., Thomashow M. F., Rittenberg S. C. Changes in cell composition and viability of Bdellovibrio bacteriovorus during starvation. Arch Microbiol. 1974 May 20;97(4):313–327. doi: 10.1007/BF00403070. [DOI] [PubMed] [Google Scholar]
  6. Ishiguro E. E. Minimum nutritional requirements for growth of host-independent derivatives of Bdellovibrio bacteriovorus strain 109 Davis. Can J Microbiol. 1974 Feb;20(2):263–264. doi: 10.1139/m74-041. [DOI] [PubMed] [Google Scholar]
  7. Johnson R. C., Livermore B. P., Walby J. K., Jenkin H. M. Lipids of parasitic and saprophytic leptospires. Infect Immun. 1970 Sep;2(3):286–291. doi: 10.1128/iai.2.3.286-291.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaneda T. Incorporation of branched-chain C6-fatty acid isomers into the related long-chain fatty acids by growing cells of Bacillus subtilis. Biochemistry. 1971 Jan 19;10(2):340–347. doi: 10.1021/bi00778a022. [DOI] [PubMed] [Google Scholar]
  9. Kessel M., Varon M. Development of bdellophage VL-1 in parasitic and saprophytic bdellovibrios. J Virol. 1973 Dec;12(6):1522–1533. doi: 10.1128/jvi.12.6.1522-1533.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LENNARZ W. J. The role of isoleucine in the biosynthesis of branched-chain fatty acids by Micrococcus lysodeikticus. Biochem Biophys Res Commun. 1961 Nov 1;6:112–116. doi: 10.1016/0006-291x(61)90395-3. [DOI] [PubMed] [Google Scholar]
  11. Linden C. D., Wright K. L., McConnell H. M., Fox C. F. Lateral phase separations in membrane lipids and the mechanism of sugar transport in Escherichia coli. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2271–2275. doi: 10.1073/pnas.70.8.2271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marr A. G., Ingraham J. L. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI. J Bacteriol. 1962 Dec;84(6):1260–1267. doi: 10.1128/jb.84.6.1260-1267.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Matin A., Rittenberg S. C. Kinetics of deoxyribonucleic acid destruction and synthesis during growth of Bdellovibrio bacteriovorus strain 109D on pseudomonas putida and escherichia coli. J Bacteriol. 1972 Sep;111(3):664–673. doi: 10.1128/jb.111.3.664-673.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. O'LEARY W. M. Studies of the utilization of C14-labeled octadecenoic acids by Lactobacillus arabinosus. J Bacteriol. 1959 Mar;77(3):367–373. doi: 10.1128/jb.77.3.367-373.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. POLAN C. E., MCNEILL J. J., TOVE S. B. BIOHYDROGENATION OF UNSATURATED FATTY ACIDS BY RUMEN BACTERIA. J Bacteriol. 1964 Oct;88:1056–1064. doi: 10.1128/jb.88.4.1056-1064.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pritchard M. A., Langley D., Rittenberg S. Effects of methotrexate on intraperiplasmic and axenic growth of Bdellovibrio bacteriovorus. J Bacteriol. 1975 Mar;121(3):1131–1136. doi: 10.1128/jb.121.3.1131-1136.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rittenberg S. C., Hespell R. B. Energy efficiency of intraperiplasmic growth of Bdellovibrio bacteriovorus. J Bacteriol. 1975 Mar;121(3):1158–1165. doi: 10.1128/jb.121.3.1158-1165.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rittenberg S. C., Langley D. Utilization of nucleoside monophosphates per Se for intraperiplasmic growth of Bdellovibrio bacteriovorus. J Bacteriol. 1975 Mar;121(3):1137–1144. doi: 10.1128/jb.121.3.1137-1144.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rittenberg S. C. Nonidentity of Bdellovibrio bacteriovorus strains 109D and 109J. J Bacteriol. 1972 Jan;109(1):432–433. doi: 10.1128/jb.109.1.432-433.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rittenberg S. C., Shilo M. Early host damage in the infection cycle of Bdellovibrio bacteriovorus. J Bacteriol. 1970 Apr;102(1):149–160. doi: 10.1128/jb.102.1.149-160.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Seideler R. J., Mandel M., Baptist J. N. Molecular heterogeneity of the Bdellovibrios: evidence of two new species. J Bacteriol. 1972 Jan;109(1):209–217. doi: 10.1128/jb.109.1.209-217.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Seidler R. J., Starr M. P., Mandel M. Deoxyribonucleic acid characterization of Bdellovibrios. J Bacteriol. 1969 Nov;100(2):786–790. doi: 10.1128/jb.100.2.786-790.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shilo M. Morphological and physiological aspects of the interaction of Bdellovibrio with host bacteria. Curr Top Microbiol Immunol. 1969;50:174–204. doi: 10.1007/978-3-642-46169-9_6. [DOI] [PubMed] [Google Scholar]
  24. Silbert D. F., Ruch F., Vagelos P. R. Fatty acid replacements in a fatty acid auxotroph of Escherichia coli. J Bacteriol. 1968 May;95(5):1658–1665. doi: 10.1128/jb.95.5.1658-1665.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  26. Steiner S., Conti S. F., Lester R. L. Occurrence of phosphonosphingolipids in Bdellovibrio bacteriovorus strain UKi2. J Bacteriol. 1973 Dec;116(3):1199–1211. doi: 10.1128/jb.116.3.1199-1211.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Varon M., Shil M. Interacton of Bdellovibrio bacteriovorus and host bacteria. I. Kinetic studies of attachment and invasion of Escherichia coli B by Bdellovibrio bacteriovorus. J Bacteriol. 1968 Mar;95(3):744–753. doi: 10.1128/jb.95.3.744-753.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Williams R. E., Wisnieski B. J., Rittenhouse H. G., Fox C. F. Utilization of fatty acid supplements by cultured animal cells. Biochemistry. 1974 Apr 23;13(9):1969–1977. doi: 10.1021/bi00706a029. [DOI] [PubMed] [Google Scholar]
  29. Wilson G., Fox C. F. Biogenesis of microbial transport systems: evidnce for coupled incorporation of newly synthesized lipids and proteins into membrane. J Mol Biol. 1971 Jan 14;55(1):49–60. doi: 10.1016/0022-2836(71)90280-4. [DOI] [PubMed] [Google Scholar]
  30. Wilson G., Rose S. P., Fox C. F. The effect of membrane lipid unsaturation on glycoside transport. Biochem Biophys Res Commun. 1970 Feb 20;38(4):617–623. doi: 10.1016/0006-291x(70)90625-x. [DOI] [PubMed] [Google Scholar]
  31. Wisnieski B. J., Williams R. E., Fox C. F. Manipulation of fatty acid composition in animal cells grown in culture. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3669–3673. doi: 10.1073/pnas.70.12.3669. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES