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. 1975 Mar;121(3):1166–1172. doi: 10.1128/jb.121.3.1166-1172.1975

Cell division suppression in the Bacillus subtilis div IC-A1 minicell-producing mutant.

N H Mendelson
PMCID: PMC246049  PMID: 803958

Abstract

Growth and division patterns of Bacillus subtilis wild-type (div IV-A1+) and minicell-producing mutant (div IV-A1) clones were studied after spore germination during microcolony development in chambers that facilitate continuous observation with a phase contrast microscope. Data obtained from 13 div IV-A1+ clones were used to derive the equation DE equals [(mum minus 17.6)/8.8], which expresses the relationship of cell divisions present in clones of various lengths. This equation was used to determine the number of divisions expected in div IV-A1 clones if the mutant clones were able to divide as often as wild-type clones. The observed number of divisions present in mutant clones was found to be only 25.27% of the number expected on the basis of this equation. Although individual div IV-A1 clones varied in the percentage of division equivalents expressed, there appeared to be no correlation between the overall clone growth rate and the number of divisions expressed. Culturing div IV-A1+ and div IV-A1 clones together in the same growth chamber revealed that there were no diffusible interactions influencing the division phenotypes of either mutant or wild-type cells. At later stages of growth, mixed microcolonies containing cells of both genotypes were formed. A length analysis of individual cells in these populations indicated that the relative division suppression of mutant compared with wild-type cells characteristic of the initial stages of clone development was maintained. It is likely, therefore, that the excessive length of minicell-producing cells (div IV-A1) is a reflection primarily of division suppression in the mutant and not simply of mislocation of division along cell length.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Breakefield X. O., Landman O. E. Temperature-sensitive divisionless mutant of Bacillus subtilis defective in the initiation of septation. J Bacteriol. 1973 Feb;113(2):985–998. doi: 10.1128/jb.113.2.985-998.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coyne S. I., Mendelson N. H. Clonal analysis of cell division in the Bacillus subtilis div IV-B1 minicell-producing mutant. J Bacteriol. 1974 Apr;118(1):15–20. doi: 10.1128/jb.118.1.15-20.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frazer A. C., Curtiss R., 3rd Production, properties and utility of bacterial minicells. Curr Top Microbiol Immunol. 1975;69:1–84. doi: 10.1007/978-3-642-50112-8_1. [DOI] [PubMed] [Google Scholar]
  5. Huang P. Y., Goodman R. N. Morphology and ultrastructure of normal rod-shaped and filamentous forms of Erwinia amylovora. J Bacteriol. 1970 Jun;102(3):862–866. doi: 10.1128/jb.102.3.862-866.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mendelson N. H., Cole R. M. Genetic regulation of cell division initiation in Bacillus subtilis. J Bacteriol. 1972 Nov;112(2):994–1003. doi: 10.1128/jb.112.2.994-1003.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mendelson N. H. Division site regulation in a temperature-sensitive mutant of Bacillus subtilis. J Bacteriol. 1972 Jul;111(1):298–300. doi: 10.1128/jb.111.1.298-300.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mendelson N. H., Reeve J. N., Cole R. M. Physiological studies of Bacillus subtilis minicells. J Bacteriol. 1974 Mar;117(3):1312–1319. doi: 10.1128/jb.117.3.1312-1319.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mendelson N. H., Reeve J. N. Growth of the Bacillus subtilis cell surface. Nat New Biol. 1973 May 9;243(123):62–64. [PubMed] [Google Scholar]
  10. Paulton R. J. Analysis of the multiseptate potential of Bacillus subtilis. J Bacteriol. 1970 Nov;104(2):762–767. doi: 10.1128/jb.104.2.762-767.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Reeve J. N., Mendelson N. H. Cell morphology of Bacillus subtilis: the effect of genetic background on the expression of a rod - gene. Mol Gen Genet. 1972;119(1):11–26. doi: 10.1007/BF00270440. [DOI] [PubMed] [Google Scholar]
  12. Reeve J. N., Mendelson N. H., Coyne S. I., Hallock L. L., Cole R. M. Minicells of Bacillus subtilis. J Bacteriol. 1973 May;114(2):860–873. doi: 10.1128/jb.114.2.860-873.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Slater M., Schaechter M. Control of cell division in bacteria. Bacteriol Rev. 1974 Jun;38(2):199–221. doi: 10.1128/br.38.2.199-221.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Teather R. M., Collins J. F., Donachie W. D. Quantal behavior of a diffusible factor which initiates septum formation at potential division sites in Escherichia coli. J Bacteriol. 1974 May;118(2):407–413. doi: 10.1128/jb.118.2.407-413.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Van Alstyne D., Simon M. I. Division mutants of Bacillus subtilis: isolation and PBS1 transduction of division-specific markers. J Bacteriol. 1971 Dec;108(3):1366–1379. doi: 10.1128/jb.108.3.1366-1379.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vörös J., Goodman R. N. Filamentous forms of Erwinia amylovora. Phytopathology. 1965 Aug;55(8):876–879. [PubMed] [Google Scholar]

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