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. 1975 May;122(2):433–436. doi: 10.1128/jb.122.2.433-436.1975

Methyltrophic enzyme distribution in Methylosinus trichosporium.

T L Weaver, P R Dugan
PMCID: PMC246075  PMID: 123917

Abstract

Key enzymes involved in the oxidation and fixation of methane by Methylosinus trichosporium were examined for localization within the bacterial cells. A differential centrifugation scheme following cell disruption was used to provide membrane and soluble fractions for the enzyme assays. All the methylotrophic enzymes examined were found to be soluble with this fractionation scheme. Electron transport involving a cytochrome c2 with absorption peaks at 416, 522, and 550 nm and oxidative phosphorylation were found in the membrane fractions. Mixed soluble and membrane fractions coupled the oxidation of emthanol and formate with cytochrome reduction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buckmire F. L., MacLeod R. A. Nutrition and metabolism of marine bacteria. XIV. On the mechanism of lysis of a marine bacterium. Can J Microbiol. 1965 Aug;11(4):677–691. doi: 10.1139/m65-091. [DOI] [PubMed] [Google Scholar]
  2. Davies S. L., Whittenbury R. Fine structure of methane and other hydrocarbon-utilizing bacteria. J Gen Microbiol. 1970 May;61(2):227–232. doi: 10.1099/00221287-61-2-227. [DOI] [PubMed] [Google Scholar]
  3. Forsberg C. W., Costerton J. W., Macleod R. A. Quantitation, chemical characteristics, and ultrastructure of the three outer cell wall layers of a gram-negative bacterium. J Bacteriol. 1970 Dec;104(3):1354–1368. doi: 10.1128/jb.104.3.1354-1368.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Forsberg C. W., Costerton J. W., Macleod R. A. Separation and localization of cell wall layers of a gram-negative bacterium. J Bacteriol. 1970 Dec;104(3):1338–1353. doi: 10.1128/jb.104.3.1338-1353.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Green D. E., Murer E., Hultin H. O., Richardson S. H., Salmon B., Brierley G. P., Baum H. Association of integrated metabolic pathways with membranes. I. Glycolytic enzymes of the red blood corpuscle and yeast. Arch Biochem Biophys. 1965 Dec;112(3):635–647. doi: 10.1016/0003-9861(65)90107-4. [DOI] [PubMed] [Google Scholar]
  6. HATEFI Y., OSBORN M. J., KAY L. D., HUENNEKENS F. M. Hydroxymethyl tetrahydrofolic dehydrogenase. J Biol Chem. 1957 Aug;227(2):637–647. [PubMed] [Google Scholar]
  7. LAW J. H., SLEPECKY R. A. Assay of poly-beta-hydroxybutyric acid. J Bacteriol. 1961 Jul;82:33–36. doi: 10.1128/jb.82.1.33-36.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEADBETTER E. R., FOSTER J. W. Incorporation of molecular oxygen in bacterial cells utilizing hydrocarbons for growth. Nature. 1959 Oct 31;184(Suppl 18):1428–1429. doi: 10.1038/1841428a0. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lawrence A. J., Quayle J. R. Alternative carbon assimilation pathways in methane-utilizing bacteria. J Gen Microbiol. 1970 Nov;63(3):371–374. doi: 10.1099/00221287-63-3-371. [DOI] [PubMed] [Google Scholar]
  11. Remsen C., Lundgren D. G. Electron microscopy of the cell envelope of Ferrobacillus ferrooxidans prepared by freeze-etching and chemical fixation techniques. J Bacteriol. 1966 Dec;92(6):1765–1771. doi: 10.1128/jb.92.6.1765-1771.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ribbons D. W., Harrison J. E., Wadzinski A. M. Metabolism of single carbon compounds. Annu Rev Microbiol. 1970;24:135–158. doi: 10.1146/annurev.mi.24.100170.001031. [DOI] [PubMed] [Google Scholar]
  13. Ribbons D. W., Michalover J. L. Methane oxidation by cell-free extracts of Methylococcus capsulatus. FEBS Lett. 1970 Nov 9;11(1):41–44. doi: 10.1016/0014-5793(70)80487-2. [DOI] [PubMed] [Google Scholar]
  14. Weaver T. L., Dugan P. R. Ultrastruct of Methylosinus trichosporium as revealed by freeze etching. J Bacteriol. 1975 Feb;121(2):704–710. doi: 10.1128/jb.121.2.704-710.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Whittenbury R., Phillips K. C., Wilkinson J. F. Enrichment, isolation and some properties of methane-utilizing bacteria. J Gen Microbiol. 1970 May;61(2):205–218. doi: 10.1099/00221287-61-2-205. [DOI] [PubMed] [Google Scholar]

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